Presentation is loading. Please wait.

Presentation is loading. Please wait.

Jill A. Poole, MD, Neil E. Alexis, PhD, Conrad Parks, BS, Amy K

Published byΆλκηστις Αλιβιζάτος Modified over 5 years ago

Similar presentations

Presentation on theme: "Jill A. Poole, MD, Neil E. Alexis, PhD, Conrad Parks, BS, Amy K"— Presentation transcript:

1 Repetitive organic dust exposure in vitro impairs macrophage differentiation and function 
Jill A. Poole, MD, Neil E. Alexis, PhD, Conrad Parks, BS, Amy K. MacInnes, BS, Martha J. Gentry-Nielsen, PhD, Paul D. Fey, PhD, Lennart Larsson, PhD, Diane Allen-Gipson, PhD, Susanna G. Von Essen, MD, MPH, Debra J. Romberger, MD  Journal of Allergy and Clinical Immunology  Volume 122, Issue 2, Pages e4 (August 2008) DOI: /j.jaci Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Cell-surface marker expression in MDMs. After 1 week, control and ODE MDMs demonstrated mCD14 and CD16 expression by means of flow cytometry. HLA-DR, CD80, and CD86 expression was diminished with ODE versus control MDMs. A, Representative histogram of 4 separate studies. B, Mean ± SEM of the MFI (n = 4). ∗Statistically significant, P < .05). Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Cell-surface marker expression in MDMs. After 1 week, control MDMs were compared with MDMs/early exposed (A; n = 3) and MDMs conditioned with heat-inactivated dust (B; n = 3), 10 ng/mL LPS (C; n = 4), 4 μg/mL PGN (D; n = 4), combination LPS plus PGN (E; n = 4), and endotoxin-depleted ODE (F; n = 3). Values are expressed as means ± SEMs of the MFI. ∗Statistically significant, P < .05. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Phagocytosis of IgG-opsonized Saccharomyces cerevisiae zymosan bioparticles. Phagocytosis was diminished in ODE-treated MDMs compared with that seen in control MDMs (n = 4, P = .001). A, Representative dot plot of rightward shift in fluorescence from 4 separate studies. B, Fold change in MFI (proportion of cells in the zymosan-exposed population at 60 minutes compared with cells exposed for 0 minutes). ∗Statistically significant, P < .05. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Phagocytosis of IgG-opsonized zymosan bioparticles in MDMs. Fold change in MFI on the y-axis (proportion of cells in the zymosan-exposed population at 60 minutes compared with cells exposed for 0 minutes) with MDMs/early ODE exposed (A; n = 3) and MDMs conditioned with heat-inactivated dust (B; n = 3), LPS (C; n = 3), PGN (D; n = 3), LPS plus PGN (E; n = 3), and endotoxin-depleted ODE (F; n = 3) is shown. ∗Statistically significant, P < .05. NS, Nonsignificant, P > .05). Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 Secretion of cytokines/chemokines in MDMs at 1 week after restimulation with ODE 1% for 5 hours. TNF-α (A), IL-6 (B), and IL-10 (C) secretion was dampened in ODE MDMs compared with that seen in control MDMs (n = 5, P < .05). CXCL8 (D) expression was not significantly altered (n = 5). Mean results are presented per 5 × 105 cells ± SEMs. ∗Statistically significant, P < .05). Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 A, Schematic representation of experimental protocol showing elutriated monocytes cultured with GM-CSF (1000 IU/mL) with and without low-concentration ODE (0.1%) for 6 days. B, Monocytes cultured with GM-CSF and ODE (0.1%) for 48 hours and washed and maintained in culture for the remaining 4 days (MDMs/early ODE exposed). Macrophages rested for 18 hours before experimental assays. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Cytokine secretion in MDMs at 1 week after restimulation with ODE (1%) for 5 hours. TNF (A) and IL-6 (B) secretion was dampened in all conditioned MDMs compared with that seen in control MDMs, except for heat-inactivated ODE MDMs (n = 3). Mean results are presented as the percentage of ODE-induced cytokine secretion in control MDMs ± SEM. ∗Statistically significant, P < .05). NS, Not significant. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

9 Cytokine secretion in MDMs at 1 week after restimulation with LPS (100 ng/mL; A and B) and PGN (40 μg/mL; C and D). TNF (Fig E2, A and C) and IL-6 (Fig E2, B and D) secretion was dampened in all conditioned MDMs compared with that seen in control MDMs (n = 3). Mean results are presented as the percentage of LPS- or PGN-induced cytokine secretion in control MDMs ± SEM. ∗Statistically significant, P < .05). Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Jill A. Poole, MD, Neil E. Alexis, PhD, Conrad Parks, BS, Amy K"

Similar presentations

Maria B. Sukkar, PhD, Shaoping Xie, PhD, Nadia M

Maria B. Sukkar, PhD, Shaoping Xie, PhD, Nadia M
Multiple-checkpoint inhibition of thymic stromal lymphopoietin–induced TH2 response by TH17-related cytokines  Sofia I. Bogiatzi, BSc, Maude Guillot-Delost,

Multiple-checkpoint inhibition of thymic stromal lymphopoietin–induced TH2 response by TH17-related cytokines  Sofia I. Bogiatzi, BSc, Maude Guillot-Delost,
Vitamin D3 treatment of vitamin D–insufficient asthmatic patients does not alter immune cell function  Brandy Reid, MS, Pierre-Olivier Girodet, MD, PhD,

Vitamin D3 treatment of vitamin D–insufficient asthmatic patients does not alter immune cell function  Brandy Reid, MS, Pierre-Olivier Girodet, MD, PhD,
Exposure to allergen and diesel exhaust particles potentiates secondary allergen- specific memory responses, promoting asthma susceptibility  Eric B. Brandt,

Exposure to allergen and diesel exhaust particles potentiates secondary allergen- specific memory responses, promoting asthma susceptibility  Eric B. Brandt,
Flow cytometry imaging identifies rare TH2 cells expressing thymic stromal lymphopoietin receptor in a “proallergic” milieu  Amanda J. Reefer, MS, Kathryn.

Flow cytometry imaging identifies rare TH2 cells expressing thymic stromal lymphopoietin receptor in a “proallergic” milieu  Amanda J. Reefer, MS, Kathryn.
The importance of being “pure” neutrophils

The importance of being “pure” neutrophils
Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 

Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 
Ambient particulate matter directs nonclassic dendritic cell activation and a mixed TH1/TH2-like cytokine response by naive CD4+ T cells  Marc A. Williams,

Ambient particulate matter directs nonclassic dendritic cell activation and a mixed TH1/TH2-like cytokine response by naive CD4+ T cells  Marc A. Williams,
The activating protein 1 transcription factor basic leucine zipper transcription factor, ATF- like (BATF), regulates lymphocyte- and mast cell–driven immune.

The activating protein 1 transcription factor basic leucine zipper transcription factor, ATF- like (BATF), regulates lymphocyte- and mast cell–driven immune.
Impaired TH17 responses in patients with chronic mucocutaneous candidiasis with and without autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy 

Impaired TH17 responses in patients with chronic mucocutaneous candidiasis with and without autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy 
The activating protein 1 transcription factor basic leucine zipper transcription factor, ATF- like (BATF), regulates lymphocyte- and mast cell–driven immune.

The activating protein 1 transcription factor basic leucine zipper transcription factor, ATF- like (BATF), regulates lymphocyte- and mast cell–driven immune.
Repeated FcεRI triggering reveals modified mast cell function related to chronic allergic responses in tissue  Jolien Suurmond, MSc, Kim L.L. Habets,

Repeated FcεRI triggering reveals modified mast cell function related to chronic allergic responses in tissue  Jolien Suurmond, MSc, Kim L.L. Habets,
IgE cross-linking impairs monocyte antiviral responses and inhibits influenza-driven TH1 differentiation  Regina K. Rowe, MD, PhD, David M. Pyle, MD,

IgE cross-linking impairs monocyte antiviral responses and inhibits influenza-driven TH1 differentiation  Regina K. Rowe, MD, PhD, David M. Pyle, MD,
Neil E. Alexis, Marianne S. Muhlebach, David B. Peden, Terry L. Noah 

Neil E. Alexis, Marianne S. Muhlebach, David B. Peden, Terry L. Noah 
Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.

Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.
Toll-like receptor 7–induced naive human B-cell differentiation and immunoglobulin production  Mark C. Glaum, MD, PhD, Shilpi Narula, MD, Decheng Song,

Toll-like receptor 7–induced naive human B-cell differentiation and immunoglobulin production  Mark C. Glaum, MD, PhD, Shilpi Narula, MD, Decheng Song,
Allergic sensitization can be induced via multiple physiologic routes in an adjuvant- dependent manner  David Dunkin, MD, M. Cecilia Berin, PhD, Lloyd.

Allergic sensitization can be induced via multiple physiologic routes in an adjuvant- dependent manner  David Dunkin, MD, M. Cecilia Berin, PhD, Lloyd.
Frank Kirstein, PhD, Natalie E

Frank Kirstein, PhD, Natalie E
Maternal house dust mite exposure during pregnancy enhances severity of house dust mite–induced asthma in murine offspring  Phoebe K. Richgels, MS, Amnah.

Maternal house dust mite exposure during pregnancy enhances severity of house dust mite–induced asthma in murine offspring  Phoebe K. Richgels, MS, Amnah.
Ramses Ilarraza, PhD, Yingqi Wu, Christopher D

Ramses Ilarraza, PhD, Yingqi Wu, Christopher D

Similar presentations

Ads by Google