1. Selected Experiments in Biochemistry BIOC4107
IUG, Spring 2015
TMZ

2. Instructor
Dr. Tarek M. Zaida
Contact
Office: Lab. Building C300
Office Phone: 2604
mobile: , WhatsApp & Viber or
Office Hours: TBD

3. Course Description
This course is designed for the students with a biochemistry major, and is intended to focus
on some carefully selected important protocols, and techniques used widely in biochemistry lab.

4. Course Objectives
1. This course aims to provide the students with an opportunity to develop their qualitative analytical skills. 2. It is expected that students upon completion of this course, will have a sound knowledge on advanced protocols applied widely in biochemistry. 3. Students will get adapted to team work. 4. Helping students developing their communication skills. 5. At the end of this course students will be familiar with “how” to prepare reports on lab activities. 6. Encouraging students to think critically and know how to solve problems.

5. Course Topics Protein Dialysis, Determination of protein pI,
Protein salting out,
Enzyme kinetics,
Aerobic respiration & anaerobic respiration,
Bradford test,
Separation of blood serum lipids by means of Thin Layer Chromatography,
Color Reactions of Plant Pigments, and
SDS-PAGE.

6. Type of Course Teaching
This is a practical course that requires:
- short lectures,
- in-lab practical experiments,
and
- Participation in different electronic learning tools.

7. Prerequisites Practical Analytical Chemistry CHEM2110
Biochemistry I BIOCA2301
Biochemistry II BIOCB2301
Practical Biochemistry I BIOCA2101
Practical Biochemistry II BIOCB2101

8. Course Timing Experiment Activity 1st Lab Activity Protein Dialysis
Report 1
2nd Lab Activity
Determination of Protein pI
Report 2
3rd Lab Activity
Protein Salting out
Report 3
4th Lab Activity
Enzyme Kinetics
Report 4 Quiz1
5th Lab Activity
Aerobic Respiration
Report 5
6th Lab Activity
Anaerobic Respiration
Report 6
7th Lab Activity
Bradford Test
Report 7 Quiz2
8th Lab Activity
Separation of Blood Serum Lipids by TLC
Report 8
9th Lab Activity
Color Reactions of Plant Pigments
Report 9
10th Lab Activity
SDS-PAGE part I
Report 10 Quiz3
11th Lab Activity
SDS-PAGE part II
Report 11

9. Evaluation
There will be different types of evaluation that will include:
1. A pre-lab quiz before each lab activity on the same day (every lab session) (total: 10 quizzes)
2. Quizzes (3) ( after every 3 lab sessions)
3. Writing Lab reports
4. Participation in the actual lab work.
5. Participation in the discussion with classmates through the electronic learning tools.

10. Course Policy
- Attending all lab sessions is a mandatory.
- Active engagement in different elearning tools (online quizzes, wikis, forum, chat, electronic reports).
- Zero tolerance for not following given instructions during lab sessions.
- Wearing the lab coat as soon as you step in the lab.
- Acting responsibly during the entire lab session.
- Team work is a must, including communication with group partners.
- Giving up reports from the previous lab session must be electronically only.
- No make ups are allowed!!!

11. Lab Safety/Health
It is my job to keep my dear students safe, and protect them against any type of danger that might be anticipated during the lab sessions due to the different kinds of hazardous materials that might be necessary for some experiments.
However students are expected to comply completely with lab’s safety rules, and will be held responsible for their own safety once they don’t follow instructor’s rules.

12. Grading Answering the pre-lab quizzes during lab sessions. 1015
Lab reports (electronically submitted) 20
Active engagement in the actual lab work
Being active in different elearning tools during the whole term
Final Quiz (in all experiments)
Practical exam (in-lab activity)

13. Following up with the Course
- Students may reach the course materials through moodle system anytime. In case of anticipating any type of problems, student may contact me at my mobile or via anytime.
How to use moodle system
- Students may go through the moodle system using guide found on the website of electronic learning , under educational resources for students.
- Students may also ask for assistance from the electronic learning center found in the IT building I400,
phone 1573.