1. Expression analysis of MC and JOINTLESS
Expression analysis of MC and JOINTLESS. A, Structures of genomic DNA and mRNA of the rin locus in the wild type and the rin mutant.
Expression analysis of MC and JOINTLESS. A, Structures of genomic DNA and mRNA of the rin locus in the wild type and the rin mutant. Exons of RIN and MC are indicated by white and gray boxes, respectively. From the wild-type genome, two normal mRNAs (RIN and MC) are transcribed, whereas from the rin mutant genome, the normal mRNA of MC and the mutant mRNA, which is a chimera of RIN and MC, are transcribed (Vrebalov et al., 2002; Kitagawa et al., 2005). B, MC and JOINTLESS expression in various organs detected by qRT-PCR analysis. Levels of transcripts of MC and JOINTLESS are shown as fold change values compared with flower pedicels of the wild type. The results are means of three biological replicates of all samples, except for the ripening fruits of the j mutant and the rin mutant, whose results are means of two biological replicates. Error bars indicate sd. SlACTIN51 (SGN-E ) was used as the reference. mRNA of flowers and flower pedicels was sampled at anthesis, and mRNA of fruit sepals and fruit pedicels was sampled at preripening stage. C, MC and JOINTLESS expression in a transformant expressing the MC antisense gene detected by qRT-PCR. Levels of transcripts are shown as fold change values compared with flower pedicels of the wild type.
Toshitsugu Nakano et al. Plant Physiol. 2011;158:
©2012 by American Society of Plant Biologists