1. Effect of CTCF deletion on thymocyte development.
Effect of CTCF deletion on thymocyte development. (A) Diagram of the Tcrb locus. Red Trbv gene segments are classified as having nearby CBEs, whereas black Trbv gene segments do not (17). Red circles denote three RC-proximal CBEs. (B) Diagram of Ctcf floxed and deleted alleles. LoxP sites are represented by triangles. PGK-puromycin (puror) and splice acceptor-lacZ (SA-LacZ) cassettes are indicated. (C) Representative flow cytometry profiles of Ctcff/f and Ctcff/fCd2-Cre thymocytes. Thymocytes were pregated on live cells and negative for other lineage markers. (D and E) Quantification of thymocyte cellularity and subpopulations as shown in (C). DN thymocyte subsets: DN1 (CD44+CD25−), DN2 (CD44+CD25+), DN3 (CD44−CD25+), and DN4 (CD44−CD25−). Means are indicated by the horizontal lines. *p < 0.05 by Student t test with Holm–Sidak correction for multiple comparisons. (F) Representative flow cytometry profiles of FDG staining in Ctcff/fCd2-Cre thymocytes. Colored outlines correspond to the identically colored boxes shown in (C). Eβ, Tcrb enhancer.
Shiwei Chen, and Michael S. Krangel ImmunoHorizons 2018;2:
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