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Compensatory role of Langerhans cells and langerin-positive dermal dendritic cells in the sensitization phase of murine contact hypersensitivity  Tetsuya.

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Presentation on theme: "Compensatory role of Langerhans cells and langerin-positive dermal dendritic cells in the sensitization phase of murine contact hypersensitivity  Tetsuya."— Presentation transcript:

1 Compensatory role of Langerhans cells and langerin-positive dermal dendritic cells in the sensitization phase of murine contact hypersensitivity  Tetsuya Honda, MD, PhD, Saeko Nakajima, MD, Gyohei Egawa, MD, Kouetsu Ogasawara, PhD, Bernard Malissen, MD, PhD, Yoshiki Miyachi, MD, PhD, Kenji Kabashima, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 125, Issue 5, Pages e2 (May 2010) DOI: /j.jaci Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Effect of DT on GFP+ cells. The CD11c+ MHC class II+ cells of the epidermis and dermis from either langerin-eGFP-DTR (Lang-GFP-DTR) mice (upper panels) or BMC mice (lower panels) were analyzed by means of flow cytometry for the expression of GFP. The frequency of GFP+ and GFP− cells in the epidermis (A) and dermis (B) before (DT−; left panels) and after (DT+; right panels) are shown. Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Role of LCs and langerin-positive dDCs in the sensitization phase of CHS. A, CHS response. B, The number of CD44+ T cells in draining lymph nodes 6 days after sensitization. C, DNBS-induced cell proliferation. LN cells (1 × 106) were cultured with or without DNBS stimulation in a 96-well plate for 72 hours; tritiated thymidine was added for the last 12 hours. D, IFN-γ level. The levels of IFN-γ in the culture medium 72 hours after incubation were measured with an ELISA kit. Data are presented as means ± SDs and are representative of 3 experiments with similar results. ∗P < .05. Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Chimerism of langerin-positive dDCs in BMC mice
Chimerism of langerin-positive dDCs in BMC mice. The CD11c+ MHC class II+ EpCAM− CD103+ langerin-positive DCs of the dermis from langerin-eGFP-DTR, BMC, or B6 mice (left panels) were analyzed by means of flow cytometry for the expression of GFP. The frequencies (percentages) of GFP+ and GFP− cells among langerin-positive dDCs are shown (right panels). APC, Allophycocyanin; PE, phycoerythrin; PECy7, phycoerythrin-Cy7; WT, Wild Type. Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Effect of DT on reconstituted langerin-positive dDCs in BMC mice
Effect of DT on reconstituted langerin-positive dDCs in BMC mice. Langerin-eGFP-DTR and BMC mice were treated with or without DT, and the CD11c+ MHC class II+ EpCAM− CD103+ and CD11c+ MHC class II+ EpCAM+ CD103− dDC subsets were analyzed by means of flow cytometry. The frequencies (percentages) of EpCAM+ CD103− and EpCAM− CD103+ cells among CD11c+ MHC class II+ DCs in the dermis are shown. APC, Allophycocyanin; PE, phycoerythrin. Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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