Presentation is loading. Please wait.

Presentation is loading. Please wait.

Analytical metrics of yeast proteome analysis using the Q-OT-qIT (Fusion) as compared with qIT-OT (Orbitrap Elite) and Q-OT (Q-Exactive) hybrids. Analytical.

Published byBarbara Dawson Modified over 5 years ago

Similar presentations

Presentation on theme: "Analytical metrics of yeast proteome analysis using the Q-OT-qIT (Fusion) as compared with qIT-OT (Orbitrap Elite) and Q-OT (Q-Exactive) hybrids. Analytical."— Presentation transcript:

1 Analytical metrics of yeast proteome analysis using the Q-OT-qIT (Fusion) as compared with qIT-OT (Orbitrap Elite) and Q-OT (Q-Exactive) hybrids. Analytical metrics of yeast proteome analysis using the Q-OT-qIT (Fusion) as compared with qIT-OT (Orbitrap Elite) and Q-OT (Q-Exactive) hybrids. The Q-OT-qIT (panel A, red) achieves identification of up to 19 peptides per second as compared with 10 with the qIT-OT system (A, black). Peak depth is likewise considerably higher on account of the faster MS2 scanning rate of the Q-OT-qIT system (B). C, plots the pace of unique yeast peptide identifications for the three instruments. For the one hour analysis, the Q-OT-qIT posts almost twice as many unique peptide identifications as compared with the qIT-OT. Similar data, except for unique proteins, is shown in D. Alexander S. Hebert et al. Mol Cell Proteomics 2014;13: © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

Download ppt "Analytical metrics of yeast proteome analysis using the Q-OT-qIT (Fusion) as compared with qIT-OT (Orbitrap Elite) and Q-OT (Q-Exactive) hybrids. Analytical."

Similar presentations

NSAF and GeneChip data have similar distribution properties.

NSAF and GeneChip data have similar distribution properties.
Correlation of log-transformed signal intensity from two Affymetrix microarray hybridizations using platelet RNA. Plotted are those probesets with an average.

Correlation of log-transformed signal intensity from two Affymetrix microarray hybridizations using platelet RNA. Plotted are those probesets with an average.
Sequence alignment of C-terminal phosphorylated plant aquaporins

Sequence alignment of C-terminal phosphorylated plant aquaporins
NSAF and GeneChip datasets have similar PLGEM parameters.

NSAF and GeneChip datasets have similar PLGEM parameters.
Phosphorylation and sequence disorder in microtubule-associated protein Tau.A, schematic illustration of the domain profile of Tau with all known phosphorylation.

Phosphorylation and sequence disorder in microtubule-associated protein Tau.A, schematic illustration of the domain profile of Tau with all known phosphorylation.
Distribution of phosphorylation sites identified in the cytosolic phosphoproteome.A, numbers of approved phosphopeptides, previously phosphorylated peptides,

Distribution of phosphorylation sites identified in the cytosolic phosphoproteome.A, numbers of approved phosphopeptides, previously phosphorylated peptides,
Now, More Than Ever, Proteomics Needs Better Chromatography

Now, More Than Ever, Proteomics Needs Better Chromatography
Percentage of proteins identified in envelope membrane extracts according to the purification method and the number of transmembrane domains. Percentage.

Percentage of proteins identified in envelope membrane extracts according to the purification method and the number of transmembrane domains. Percentage.
Plasma CEP adducts and autoantibodies by donor age.

Plasma CEP adducts and autoantibodies by donor age.
Frequency distribution of the GRAVY of the theoretical proteins (open bars) and of 110 genes encoding proteins identified on a 2-D electrophoresis gel,

Frequency distribution of the GRAVY of the theoretical proteins (open bars) and of 110 genes encoding proteins identified on a 2-D electrophoresis gel,
Top-down protein identification.

Top-down protein identification.
Schematic of MS1 filtering.

Schematic of MS1 filtering.
Distributions of the ELDP values and Mascot scores for all protein identifications.a, frequency of ELDP value returned by correct (gray bars) and incorrect.

Distributions of the ELDP values and Mascot scores for all protein identifications.a, frequency of ELDP value returned by correct (gray bars) and incorrect.
Sequence similarity cluster of SPOUT methyltransferases.

Sequence similarity cluster of SPOUT methyltransferases.
Significantly enriched phosphorylation motifs from up-regulated phosphopeptides by Motif-X analysis. Significantly enriched phosphorylation motifs from.

Significantly enriched phosphorylation motifs from up-regulated phosphopeptides by Motif-X analysis. Significantly enriched phosphorylation motifs from.
Relative abundance of proteins identified in MALDI IMS

Relative abundance of proteins identified in MALDI IMS
Comparison of ROC plots for the PMF quality metrics using test dataset 2 (44 C. difficile proteins).a, ROC curves for coverage (open squares), MC (solid.

Comparison of ROC plots for the PMF quality metrics using test dataset 2 (44 C. difficile proteins).a, ROC curves for coverage (open squares), MC (solid.
Bottom-up proteomic characterization of MALDI IMS samples.

Bottom-up proteomic characterization of MALDI IMS samples.
Representative example of LAXIC performance for complex plant phosphoproteome. Representative example of LAXIC performance for complex plant phosphoproteome.A.

Representative example of LAXIC performance for complex plant phosphoproteome. Representative example of LAXIC performance for complex plant phosphoproteome.A.
Results from the Morris water task.

Results from the Morris water task.

Similar presentations

Ads by Google