1. a b NHS/RA Serum IL-17FF pg/mL % Recovery n=5 SD
Normal CSF IL-17FF pg/mL
0.1
99.8
20.86
6.26
114.2
4.02
0.2
107.2
10.96
0.4
94.4
5.86
0.3
105.8
8.76 1
97.8
5.17 10
104
4.95
NHS/RA Serum IL-17AA pg/mL
Normal CSF IL-17AA pg/mL
120.2
9.68
118
5.61
105.4
8.17
95.4
7.54 88
7.68
102
2.35
Table S2. Average percent recovery of recombinant IL-17AA and IL-17FF in analyte-depleted serum (a) and CSF (b).
(IL-17AA: 4 NHS and 1 RA. IL-17FF 3 NHS and 2 RA). Serum from healthy and RA patients met the criteria of ± 20% recovery from nominal at all levels tested. Given the assay LOD of pg/mL and the acceptable recovery data, the LLOQ in matrix was set at 0.1 pg/mL. Accuracy in CSF was also within ± 20% of the nominal spike