1. The start of a new era in bile physiology
Ronald Oude Elferink 
Journal of Hepatology 
Volume 41, Issue 6, Pages (December 2004)
DOI: /j.jhep
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2. Fig. 1
The time course of daunomycin transport in liver membrane vesicles. Daunomycin transport in CMV (circles) and SMV (squares) was measured in a medium containing 10mM Hepes-Tris buffer, pH 7.4, 0.2mM CaCl2, 10mM MgCl2, 12μM [3H] daunomycin, and ATP-regenerating system (3mM creatine, 3.6μg of creatine phosphokinase) with 1.2mM ATP (closed symbols) or with-out ATP (open symbols) in a total volume of 0.1ml. The medium was preincubated for 10min at 37°C, and the reaction was started by addition of 20μl membrane vesicles (38–70μg of protein). At appropriate times, aliquots (20μl) were removed and filtered, and radioactivity associated with vesicles was measured as described under ‘experimental procedures.’ Each point represents the mean value of triplicate determinations. Inset shows the effect of medium osmolarity on daunomycin transport by CMV. The [3H] daunomycin was measured in CMV which were treated for 60min at 25°C with 0–5M raffinose. Transport was performed in a medium as described and adjusted to the osmolality of the vesicle buffer. Vesicle-associated daunomycin after 1min of incubation was measured in the presence (closed circles) or absence (open circles) of ATP. Each point represents the mean value of triplicate determinations. Figure reproduced with permission.
Journal of Hepatology  , DOI: ( /j.jhep )
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