1. Stains

2. Negative Stain

3. Cheek Cells Without Stain

4. Cheek Cells with Methylene Blue

5. Differential Stains: Gram Stain
Color of
Gram +
Gram –
Primary stain:
Crystal violet
Purple
Mordant:
Iodine
Decolorizing agent:
Alcohol-acetone
Colorless
Counterstain:
Safranin
Red

6. Gram Positive and Gram Negative Bacteria

7. Prokaryotic Cell Drawing

8. Outer membrane
Peptidoglycan
GRAM NEGATIVE
GRAM POSITIVE

9. Acid-Fast and Non Acid-Fast

10. Endospore Stain: Ziehl-Neelsen

11. Figure 3.12a-c

12. Endospore Stain

13. Capsule Stain

14. Capsule Stain

15. Figure 3.12a-c

16. Flagella Stain

17. Flagella Stain

18. Figure 3.12a-c

19. Stain Comparisons G+ (PURPLE) G – (PINK) ACID FAST SPORE STAIN
PRIMARY STAIN
Crystal violet
Carbol Fuschia
Malachite green
MORDANT
Iodine
Heat
DECOLORIZER
ETOH or acetone:
Cell is purple
Cell is clear
Acid alcohol
Water
COUNTER-STAIN
Safranin:
Cell is pink
Methylene Blue
Spores are green