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FSS-i 3 DNA expert system suite
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DNA Profiling Sample e.g. hair, skin
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DNA Profiling Sample e.g. hair, skin Process sample to obtain raw data
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DNA Profiling Sample e.g. hair, skin Process sample to obtain raw data Produce a profile of the DNA
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DNA Profiling Sample e.g. hair, skin Process sample to obtain raw data Produce a profile of the DNA Store profile in NDIS (National DNA Index System) or match to existing profile
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Expert System profiling motivation Sample & data processing already heavily automated
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Expert System profiling motivation Sample & data processing already heavily automated Sample data analysis; DNA profiling is the bottle neck.
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Expert System profiling motivation Sample & data processing already heavily automated Sample data analysis; DNA profiling is the bottle neck. Two independent data reviews required to upload a profile to NDIS
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Expert System profiling motivation Sample & data processing already heavily automated Sample data analysis; DNA profiling is the bottle neck. Two independent data reviews required to upload a profile to NDIS Save enormous amounts of review time
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FSS-i 3 Expert System FSS: Forensic Science Service of the UK
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FSS-i 3 Expert System FSS: Forensic Science Service of the UK Developed a software suit called FSS-i 3 –6 years of R&D and validation
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FSS-i 3 Expert System FSS: Forensic Science Service of the UK Developed a software suit called FSS-i 3 –6 years of R&D and validation Designed by forensic scientists for forensic scientists
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FSS-i 3 Expert System FSS: Forensic Science Service of the UK Developed a software suit called FSS-i 3 –6 years of R&D and validation Designed by forensic scientists for forensic scientists Made available to forensic community –USA, Canada, Europe, Australia, New Zealand
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FSS-i 3 Expert System FSS: Forensic Science Service of the UK Developed a software suit called FSS-i 3 –6 years of R&D and validation Designed by forensic scientists for forensic scientists Made available to forensic community –USA, Canada, Europe, Australia, New Zealand Full Audit system
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i-STRess The core DNA interpretation tool
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i-STRess The core DNA interpretation tool Produces single source DNA profiles using configurable rules & filters
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i-STRess The core DNA interpretation tool Produces single source DNA profiles using configurable rules & filters Detect multi-person DNA mixtures within a profile & export to i-STReam…
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i-STReam Unique functionality to resolve two person DNA mixtures
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i-STReam Unique functionality to resolve two person DNA mixtures Allows previously unusable DNA profiles to be uploaded to DNA databases
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i-ntegrity DNA samples/data can become contaminated: accidental DNA mixture
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i-ntegrity DNA samples/data can become contaminated: accidental DNA mixture System flags possible profile contaminations for further review
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i-ntegrity DNA samples/data can become contaminated: accidental DNA mixture System flags possible profile contaminations for further review Saves time: no more manual reviews of profiles
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FSS-i 3 Validation FSS-i 3 developed from internal FSS expert systems –6 years of testing
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FSS-i 3 Validation FSS-i 3 developed from internal FSS expert systems –6 years of testing 1 million+ profiles uploaded to UK National DNA DB
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FSS-i 3 Validation FSS-i 3 developed from internal FSS expert systems –6 years of testing 1 million+ profiles uploaded to UK National DNA DB FSS-i 3 validation project: –70 000 previously analyzed samples –Minor discrepancies in 0.03% of samples & each of these samples were flagged by the system for further review
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Technology used in FSS-i 3 Strategies for implementing rules: unknown
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Technology used in FSS-i 3 Strategies for implementing rules: unknown User optimization: 20+ rules settings
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Technology used in FSS-i 3 Strategies for implementing rules: unknown User optimization: 20+ rules settings Designed to run on stand-alone Pentium 3 PCs
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Design & Development of FSS-i 3 Development time: six years
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Design & Development of FSS-i 3 Development time: six years The FSS developers who designed the FSS-i3 suite are, first and foremost, DNA scientists who understand the problems forensic scientists face (1)
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Design & Development of FSS-i 3 Development time: six years The FSS developers who designed the FSS-i3 suite are, first and foremost, DNA scientists who understand the problems forensic scientists face (1) i-STReam is based on scientific theory developed by Dr Peter Gill and Dr Tim Clayton (1)
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Conclusions: FSS-i 3 Consistent profiling of samples
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Conclusions: FSS-i 3 Consistent profiling of samples Ability to interpret mixed DNA profiles
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Conclusions: FSS-i 3 Consistent profiling of samples Ability to interpret mixed DNA profiles Check for contamination
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Conclusions: FSS-i 3 Consistent profiling of samples Ability to interpret mixed DNA profiles Check for contamination Significant reduction in time spent on the above, due to automation
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References (1) http://www.forensic.gov.uk/forensic_t/i3/index.htmhttp://www.forensic.gov.uk/forensic_t/i3/index.htm (2) http://www.promega.com/profiles/802/profilesindna_802_08.pdfhttp://www.promega.com/profiles/802/profilesindna_802_08.pdf (3) http://forensics.marshall.edu/NEST/Nest%20PDFs/Documents/NIJEvalExpe rtSys.pdf http://forensics.marshall.edu/NEST/Nest%20PDFs/Documents/NIJEvalExpe rtSys.pdf (4) http://www.promega.com/profiles/1001/profilesindna_1001_11.pdfhttp://www.promega.com/profiles/1001/profilesindna_1001_11.pdf (5) http://www.kathyreichs.com/dnaprofiling.htmhttp://www.kathyreichs.com/dnaprofiling.htm (6) http://www.biotechnologyonline.gov.au/human/dnaprofile.cfmhttp://www.biotechnologyonline.gov.au/human/dnaprofile.cfm
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