1. Correlation between DNA methylation and gene expression changes in T2D islets.
Correlation between DNA methylation and gene expression changes in T2D islets. (A) Out of 41 genes that are differentially methylated and differentially expressed (adjusted P<0.05; cf. Supplementary Table S4), 34 genes, ≈83%, show anticorrelation between methylation and expression (33 are hypomethylated and overexpressed, PER2 is hypermethylated and its expression is decreased in T2D islets; Supplementary Table S5) while 7 (≈17%) show a positive correlation. (B) Boxplots of DNA methylation levels and gene expression in 41 genes showing differential expression in T2D islets. Prevalent promoter hypomethylation is accompanied by overexpression of the associated genes. (C) Three exemplative genes with indicated promoters, methylation probes and expression probes. ALDH3B1 methylation is measured at promoter #2 and is correlated with expression data of a common exon that is present in all but one of the ALDH3B1 mRNAs. In case of hypomethylation uniquely occurring at promoter #2 (promoters #1 & #3 are not interrogated by the Infinium assay), the effect of this promoter hypomethylation on gene expression would be abated by the unchanged expression of isoforms transcribed from the other two promoters. In the case of PER2, methylation at promoter #1 and expression of the long isoform is assessed. *There are several Affymetrix probes for CASP10 that demonstrate differential expression but fail to reach the significance criterion of P<0.05 (BH‐corrected P of Student's t‐test; cf. Supplementary Table S4).
Michael Volkmar et al. EMBO J. 2012;31:
© as stated in the article, figure or figure legend