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Volume 61, Issue 1, Pages S106-S109 (January 2002)

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1 Volume 61, Issue 1, Pages S106-S109 (January 2002)
Effects of amino acids and glucose on mesangial cell aminopeptidase a and angiotensin receptors  Robert F. Chouinard, Rick L. Meek, Sheryl K. Cooney, Katherine R. Tuttle  Kidney International  Volume 61, Issue 1, Pages S106-S109 (January 2002) DOI: /j s1106.x Copyright © 2002 International Society of Nephrology Terms and Conditions

2 Figure 1 Effects of high levels of amino acids (HA) and glucose (HG) on aminopeptidase A (APA) mRNA expression. Confluent MC were serum deprived for 48 hours then subjected to HA or HG conditions for 48 hours. Total RNA was isolated and mRNA was assessed by RT-PCR. The mRNA values are expressed in arbitrary units relative to βbgr;-actin for each sample. Bars indicate mean ± SEM, *P < 0.05, N = 8. Kidney International  , S106-S109DOI: ( /j s1106.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

3 Figure 2 Effects of high levels of amino acids (HA) and glucose (HG) on angiotensin type 1 receptor (AT1R) mRNA expression. Confluent MC were serum deprived for 48 hours then subjected to HA or HG conditions for 48 hours. Total RNA was isolated and mRNA was assessed by RT-PCR. The mRNA values are expressed in arbitrary units relative to βbgr;-actin for each sample. Bars indicate mean ± SEM, *P < 0.05, N = 8. Kidney International  , S106-S109DOI: ( /j s1106.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

4 Figure 3 Effects of high levels of amino acids (HA) and glucose (HG) on angiotensin type 2 receptor (AT2R) mRNA expression. Confluent MC were serum deprived for 48 hours then subjected to HA or HG conditions for 48 hours. Total RNA was isolated and mRNA was assessed by RT-PCR. The mRNA values are expressed in arbitrary units relative to βbgr;-actin for each sample. Bars indicate mean ± SEM, *P < 0.05, N = 6. Kidney International  , S106-S109DOI: ( /j s1106.x) Copyright © 2002 International Society of Nephrology Terms and Conditions


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