1. IL-13 Signals Independent of IL-4 Receptor-Alpha Chain to Drive Ovalbumin-Induced Dermatitis 
J. Claire Hoving, Natalie E. Nieuwenhuizen, Georgia Schäfer, Arieh A. Katz, Frank Brombacher 
Journal of Investigative Dermatology 
Volume 136, Issue 6, Pages (June 2016)
DOI: /j.jid
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2. Figure 1
IL-13 causes dermatitis in IL-4Rα–deficient mice. (a) Hematoxylin and eosin stained skin sections. Original magnification ×200. (b) Epidermal thickness from two individual experiments. Data represent at least 2 individual experiments (n = 5–6). ∗∗P < 0.01, ∗∗∗P < versus BALB/c PBS-treated mice; ##P < 0.01, ###P < for IL-4Rα-/- OVA versus IL-4Rα-/-/IL-13-/- or IL-4Rα-/-/IL-13Tg OVA mice or IL-4Rα-/-/IL-13-/- versus IL-4Rα-/-/IL-13Tg mice. Scale bar = 100 μm. Values are given as mean ± standard error of the mean, and significant differences were determined using unpaired two-tailed Student t tests (GraphPad Prism version 4, GraphPad Software, San Diego, CA). P < 0.05 was considered significant. IL-4Rα, IL-4 receptor-alpha; ns, not significant; OVA, ovalbumin; PBS, phosphate buffered saline.
Journal of Investigative Dermatology  , DOI: ( /j.jid )
Copyright © 2016 The Authors Terms and Conditions

3. Figure 2
IL-13 is important in inducing the allergic phenotype during dermatitis. (a) IL-13 production in lymph nodes. (b) Total serum IgE level. Cell infiltration into the skin showing (c) eosinophils and (d) mast cells (including histology). Data represent 2 or more individual experiments (n = 5–6). ∗∗P < 0.01 and ∗∗∗P < versus BALB/c PBS-treated mice. (c) and (d) represent pooled data from 2 independent experiments, * and *** represent IL-4Rα-/-/IL-13-/- vs. IL-4Rα-/-/IL-13Tg. Scale bar left panel = 100 μm and scale bar right panel = 20 μm. Values are given as mean ± standard error of the mean, and significant differences were determined using unpaired two-tailed Student t tests (GraphPad Prism version 4, GraphPad Software, San Diego, CA). Values of p <0.05 were considered significant. IL-4Rα, IL-4 receptor-alpha; ND, not determined; ns, not significant; ova, ovalbumin; PBS, phosphate buffered saline.
Journal of Investigative Dermatology  , DOI: ( /j.jid )
Copyright © 2016 The Authors Terms and Conditions