1. Shared components define the ‘ancient’ phagosome.
Shared components define the ‘ancient’ phagosome. (A) Predicted orthologs of phagosome proteins of Dictyostelium, Drosophila, and mouse were analyzed by BLAST against the two other species and mapped according to −Log10(e‐value), where 0 indicates the absence of an ortholog and 181 a perfect alignment. Four distinct groups of proteins are highlighted for each organism: (1) a set of orthologs shared by the three organisms defining the ‘ancient’ phagosome (blue data points outside the x and y axes), (2 and 3) groups of conserved proteins shared only between the plotted organism and one of the two others found on the x or y axis (green or red data points), and (4) a set of proteins unique to the plotted organism (purple data points at the origin of the graph). As several data points may overlay in the scatter plot, a histogram below each plot reports the relative distribution of proteins among the four distinct groups of proteins. (B) Annotation of a function to each protein of the mouse proteome highlights the level of conservation of relevant phagosome functions among the three organisms. Although a large proportion of the proteins associated with functions such as ‘membrane trafficking,’ ‘small GTPases,’ and ‘cytoskeleton’ are majorly shared by the three organisms, some like ‘membrane receptors’ and ‘immunity’ are more specific to mouse and Drosophila phagosomes. See also Supplementary Figure S1 and Supplementary Datasets 4–6.
Jonathan Boulais et al. Mol Syst Biol 2010;6:423
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