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Potentiated Hsp104 variants rescue toxicity of ALS-linked FUS mutants.

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Presentation on theme: "Potentiated Hsp104 variants rescue toxicity of ALS-linked FUS mutants."— Presentation transcript:

1 Potentiated Hsp104 variants rescue toxicity of ALS-linked FUS mutants.
Potentiated Hsp104 variants rescue toxicity of ALS-linked FUS mutants. (A) Selected strains from B expressing the indicated constructs were induced for 5 h, lysed and then immunoblotted for the specified proteins. PGK serves as a loading control. (B) Δhsp104 yeast were co-transformed with the indicated pAG413GAL-FUS variant and the indicated pAG416GAL-Hsp104 variant or vector control. Strains were serially diluted fivefold and spotted on glucose (off) or galactose (on) medium. (C) Fluorescence microscopy of cells co-expressing FUS-GFP or FUSR521C-GFP and Hsp104WT, Hsp104A503V, Hsp104V426L or vector (416GAL). (D) FUS aggregation was quantified by counting the number of cells containing 0 or 1 or more foci. Values represent means±s.e.m. (n=2–3). Meredith E. Jackrel, and James Shorter Dis. Model. Mech. 2014;7: © Published by The Company of Biologists Ltd

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