1. Ischemic preconditioning enhances recovery of isolated rat lungs after hypothermic preservation 
Roland L Featherstone, PhD, David J Chambers, PhD, Frank J Kelly, PhD 
The Annals of Thoracic Surgery 
Volume 69, Issue 1, Pages (January 2000)
DOI: /S (99)

2. Fig 1
Experimental protocols for study 1 and study 2. Open bars indicate normothermic perfusion (before and after storage), striped bars indicate ischemic storage at 4°C in bicarbonate buffer (BB), and filled bars indicate preconditioning (cessation of ventilation or perfusion as indicated). All durations are given in minutes. Arrows indicate commencement of ischemic periods and reperfusion after storage.
The Annals of Thoracic Surgery  , DOI: ( /S (99) )

3. Fig 2
Pulmonary compliance (A) and vascular resistance (B) during 40 minutes of reperfusion with sanguineous bicarbonate buffer in isolated rat lungs after no storage (•), 6 hours of storage in bicarbonate buffer (■), or 6 hours of storage in bicarbonate buffer after 5 minutes of preconditioning (○), 10 minutes of preconditioning (▵), or two periods of 5 minutes of preconditioning (□). Data are expressed as mean ± standard error of the mean, n = 10 lungs per group. Some error bars have been omitted for clarity. ∗Significantly different from tissues stored for 6 hours in bicarbonate buffer after 40 minutes of reperfusion; †significantly different from unstored tissues after 60 minutes of perfusion (Dunnett’s test, p < 0.05).
The Annals of Thoracic Surgery  , DOI: ( /S (99) )

4. Fig 3
Difference in perfusate pH leaving and entering isolated rat lungs (A) and wet to dry weight ratio of rat lungs (B), after (1) no storage, (2) 6 hours of storage in modified bicarbonate buffer followed by 40 minutes of reperfusion with sanguineous bicarbonate buffer, or 6 hours of storage in modified bicarbonate buffer and 40 minutes of reperfusion after (3) 5 minutes of preconditioning, (4) 10 minutes of preconditioning, or (5) two periods of 5 minutes of preconditioning. Data are expressed as mean + standard error of the mean, n = 8 to 10 lungs per group. Some error bars have been omitted for clarity. ∗Significantly different from tissues stored for 6 hours in bicarbonate buffer after 40 minutes of reperfusion; †significantly different from unstored tissues after 60 minutes of perfusion (Dunnett’s test, p < 0.05).
The Annals of Thoracic Surgery  , DOI: ( /S (99) )

5. Fig 4
Pulmonary compliance during 40 minutes of reperfusion with sanguineous bicarbonate buffer in isolated rat lungs after no storage (•), 6 hours of storage in bicarbonate buffer (■), or 6 hours of storage in bicarbonate buffer after two periods of 5 minutes of preconditioning (□), two periods of 5 minutes of preconditioning with continued ventilation (○), or continued perfusion (▵). Data are expressed as mean ± standard error of the mean, n = 5 to 7 lungs per group. Some error bars have been omitted for clarity. ∗Significantly different from tissues stored for 6 hours in bicarbonate buffer after 40 minutes of reperfusion; †significantly different from unstored tissues after 60 minutes of perfusion (Dunnett’s test, p < 0.05).
The Annals of Thoracic Surgery  , DOI: ( /S (99) )