Presentation is loading. Please wait.

Presentation is loading. Please wait.

Thaned Kangsamaksin, Rebecca J. Morris 

Published byJasper Carson Modified over 5 years ago

Similar presentations

Presentation on theme: "Thaned Kangsamaksin, Rebecca J. Morris "— Presentation transcript:

1 Bone Morphogenetic Protein 5 Regulates the Number of Keratinocyte Stem Cells from the Skin of Mice 
Thaned Kangsamaksin, Rebecca J. Morris  Journal of Investigative Dermatology  Volume 131, Issue 3, Pages (March 2011) DOI: /jid Copyright © 2011 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Localization of bone morphogenetic protein 5 (Bmp5) in mouse hair follicles and interfollicular epidermis of wild-type but not mutant mouse skin. Adult mouse skin was collected for paraffin-embedded histological sections for (a) C57BL/6, (b) BALB/c, (c) wild-type, and (d) homozygous short-ear mutant mice at ∼7 to 8 weeks of age, and BMP5 immunohistochemistry was performed. B, hair follicle bulge; D, dermis; E, epidermis; HF, hair follicle; SG, sebaceous gland. Note the intense staining in the hair follicle and in an occasional interfollicular cell of the wild-type epithelium. In the homozygous short-ear mutant skin, Bmp5 staining was consistently absent from hair follicles and interfollicular epidermis. Scale bars=30μm. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2011 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Quantification of mouse keratinocyte colonies. Keratinocytes were isolated from 7- to 8-week-old mice. (a) The photographs of the colony assays in 60-mm dishes are shown. The (b) number and (c) size of mouse keratinocyte colonies from all genotypes were quantified after 2 weeks of cultivation on irradiated 3T3 fibroblasts as a feeder layer. Note that the number of colonies is significantly less in the homozygous short-ear mutant mice, whereas colony number and size were relatively greater in the heterozygous and wild-type congenic littermates. Average number of colonies±SD. *P-value <0.003, N=3. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2011 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Quantification of label-retaining cells (LRCs) in mouse epidermis. (a–d) Immunohistographs for LRCs are shown. Mice received twice-daily (1000 and 1700hours) subcutaneous injections of 50μg per g body weight of BrdU at postnatal days D3, D4, and D5. After 8–10 weeks, dorsal skins were collected and fixed with 10% formalin for at least 24hours. Cells retaining the label are identified as LRCs. (e) The bar graph shows the quantification of labeled cells in the interfollicular epidermis and hair follicles combined. Labeled cells were counted in the same region of the dorsal skin for every 500 cells. Mean±SD. *P-value <0.05. N=3. Scale bars=30μm. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2011 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 Exogenous recombinant human bone morphogenetic protein 5 (BMP5) increases keratinocyte colony size and number. (a) To determine the functional significance of Bmp5 on keratinocyte colony formation, we treated wild-type and short-ear keratinocytes cultured on 3T3 feeder layers with different concentrations of recombinant human BMP5 (0.01 and 0.1μgml–1). The recombinant peptide was added to the medium that was changed every other day up to 2 weeks of culture. Note that exogenous BMP5 added to culture increased the number and size of colonies in keratinocytes from all three genotypes. (b) The photographs of the colony assays each correspond to the bar graph directly above. Average number of colonies±SD. *P-value <0.05. N=3. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2011 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 Bone morphogenetic protein 5 (Bmp5) regulates the number of tumors in mice in the two-stage skin carcinogenesis model. (a) The bar graphs represent the average number of skin tumor across all genotypes. Mouse skin was initiated with 200nmol of 7,12-dimethylbenz[a]anthracene (DMBA) in 200μl of spectral grade acetone when the mice were 7 weeks of age. After 1 week, there was twice-weekly promotion with 17nmol of 12-O-tetradecanoylphorbol-13-acetate (TPA) in 200μl of acetone. Skin tumors were counted weekly for 20 weeks. Note that Bmp5 status was associated with the number of skin tumors, with short-ear mutant and BALB/c mice developing more skin tumors than heterozygous and wild-type littermates, or C57BL/6J wild-type mice. Wild-type C57BL/6 and BALB/c mice were used, respectively, as low- and high- tumor controls. (b) The marked-line chart shows the appearance of tumors across all genotypes. Mean±SD. *P-value <0.05. N=5. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2011 The Society for Investigative Dermatology, Inc Terms and Conditions

Download ppt "Thaned Kangsamaksin, Rebecca J. Morris "

Similar presentations

Keratinocyte Stem Cells but Not Melanocyte Stem Cells Are the Primary Target for Radiation-Induced Hair Graying  Hitomi Aoki, Akira Hara, Tsutomu Motohashi,

Keratinocyte Stem Cells but Not Melanocyte Stem Cells Are the Primary Target for Radiation-Induced Hair Graying  Hitomi Aoki, Akira Hara, Tsutomu Motohashi,
Bone Morphogenetic Protein Signaling Suppresses Wound-Induced Skin Repair by Inhibiting Keratinocyte Proliferation and Migration  Christopher J. Lewis,

Bone Morphogenetic Protein Signaling Suppresses Wound-Induced Skin Repair by Inhibiting Keratinocyte Proliferation and Migration  Christopher J. Lewis,
Psoriatic Inflammation Facilitates the Onset of Arthritis in a Mouse Model  Mayuko Yamamoto, Kimiko Nakajima, Mikiro Takaishi, Shun Kitaba, Yasuhiro Magata,

Psoriatic Inflammation Facilitates the Onset of Arthritis in a Mouse Model  Mayuko Yamamoto, Kimiko Nakajima, Mikiro Takaishi, Shun Kitaba, Yasuhiro Magata,
Ephrin-A2 and Ephrin-A5 Are Important for the Functional Development of Cutaneous Innervation in a Mouse Model  Dulharie T. Wijeratne, Jennifer Rodger,

Ephrin-A2 and Ephrin-A5 Are Important for the Functional Development of Cutaneous Innervation in a Mouse Model  Dulharie T. Wijeratne, Jennifer Rodger,
Ceramide Synthase 4 Regulates Stem Cell Homeostasis and Hair Follicle Cycling  Franziska Peters, Susanne Vorhagen, Susanne Brodesser, Kristin Jakobshagen,

Ceramide Synthase 4 Regulates Stem Cell Homeostasis and Hair Follicle Cycling  Franziska Peters, Susanne Vorhagen, Susanne Brodesser, Kristin Jakobshagen,
Activated Kras Alters Epidermal Homeostasis of Mouse Skin, Resulting in Redundant Skin and Defective Hair Cycling  Anandaroop Mukhopadhyay, Suguna R.

Activated Kras Alters Epidermal Homeostasis of Mouse Skin, Resulting in Redundant Skin and Defective Hair Cycling  Anandaroop Mukhopadhyay, Suguna R.
Unprocessed Interleukin-36α Regulates Psoriasis-Like Skin Inflammation in Cooperation With Interleukin-1  Katelynn A. Milora, Hangfei Fu, Ornella Dubaz,

Unprocessed Interleukin-36α Regulates Psoriasis-Like Skin Inflammation in Cooperation With Interleukin-1  Katelynn A. Milora, Hangfei Fu, Ornella Dubaz,
Inactivation of the Vitamin D Receptor Enhances Susceptibility of Murine Skin to UV- Induced Tumorigenesis  Tara I. Ellison, Molly K. Smith, Anita C. Gilliam,

Inactivation of the Vitamin D Receptor Enhances Susceptibility of Murine Skin to UV- Induced Tumorigenesis  Tara I. Ellison, Molly K. Smith, Anita C. Gilliam,
Upregulation of Inflammatory Cytokines and Oncogenic Signal Pathways Preceding Tumor Formation in a Murine Model of T-Cell Lymphoma in Skin  Xuesong Wu,

Upregulation of Inflammatory Cytokines and Oncogenic Signal Pathways Preceding Tumor Formation in a Murine Model of T-Cell Lymphoma in Skin  Xuesong Wu,
Figure 3. Effect of HPD on basal cell proliferation in mouse skin

Figure 3. Effect of HPD on basal cell proliferation in mouse skin
Ectodysplasin A Pathway Contributes to Human and Murine Skin Repair

Ectodysplasin A Pathway Contributes to Human and Murine Skin Repair
Overexpression of Hedgehog Signaling Is Associated with Epidermal Tumor Formation in Vitamin D Receptor–Null Mice  Arnaud E. Teichert, Hashem Elalieh,

Overexpression of Hedgehog Signaling Is Associated with Epidermal Tumor Formation in Vitamin D Receptor–Null Mice  Arnaud E. Teichert, Hashem Elalieh,
Human Papillomavirus E6/E7 Oncogenes Promote Mouse Ear Regeneration by Increasing the Rate of Wound Re-epithelization and Epidermal Growth  Concepción.

Human Papillomavirus E6/E7 Oncogenes Promote Mouse Ear Regeneration by Increasing the Rate of Wound Re-epithelization and Epidermal Growth  Concepción.
Impaired Keratinocyte Proliferative and Clonogenic Potential in Transgenic Mice Overexpressing 14-3-3σ in the Epidermis  Francesca Cianfarani, Silvia.

Impaired Keratinocyte Proliferative and Clonogenic Potential in Transgenic Mice Overexpressing σ in the Epidermis  Francesca Cianfarani, Silvia.
Protective Effect of Kit Signaling for Melanocyte Stem Cells against Radiation-Induced Genotoxic Stress  Hitomi Aoki, Akira Hara, Tsutomu Motohashi, Takahiro.

Protective Effect of Kit Signaling for Melanocyte Stem Cells against Radiation-Induced Genotoxic Stress  Hitomi Aoki, Akira Hara, Tsutomu Motohashi, Takahiro.
Overexpression of PRAS40T246A in the Proliferative Compartment Suppresses mTORC1 Signaling, Keratinocyte Migration, and Skin Tumor Development  Okkyung.

Overexpression of PRAS40T246A in the Proliferative Compartment Suppresses mTORC1 Signaling, Keratinocyte Migration, and Skin Tumor Development  Okkyung.
Hair Cycle Resting Phase Is Regulated by Cyclic Epithelial FGF18 Signaling  Miho Kimura-Ueki, Yuko Oda, Junko Oki, Akiko Komi-Kuramochi, Emi Honda, Masahiro.

Hair Cycle Resting Phase Is Regulated by Cyclic Epithelial FGF18 Signaling  Miho Kimura-Ueki, Yuko Oda, Junko Oki, Akiko Komi-Kuramochi, Emi Honda, Masahiro.
Wanglong Qiu, Xiaojun Li, Hongyan Tang, Alicia S. Huang, Andrey A

Wanglong Qiu, Xiaojun Li, Hongyan Tang, Alicia S. Huang, Andrey A
Atypical Protein Kinase C Isoform, aPKCλ, Is Essential for Maintaining Hair Follicle Stem Cell Quiescence  Shin-Ichi Osada, Naoko Minematsu, Fumino Oda,

Atypical Protein Kinase C Isoform, aPKCλ, Is Essential for Maintaining Hair Follicle Stem Cell Quiescence  Shin-Ichi Osada, Naoko Minematsu, Fumino Oda,
Enrichment for Living Murine Keratinocytes from the Hair Follicle Bulge with the Cell Surface Marker CD34  Rebecca J. Morris, Carl D. Bortner, George.

Enrichment for Living Murine Keratinocytes from the Hair Follicle Bulge with the Cell Surface Marker CD34  Rebecca J. Morris, Carl D. Bortner, George.

Similar presentations

Ads by Google