1. Anaesthesia with propofol decreases FMLP-induced neutrophil respiratory burst but not phagocytosis compared with isoflurane 
J. Heine, K. Jaeger, A. Osthaus, N. Weingaertner, S. Münte, S. Piepenbrock, M. Leuwer 
British Journal of Anaesthesia 
Volume 85, Issue 3, Pages (September 2000)
DOI: /bja/
Copyright © 2000 British Journal of Anaesthesia Terms and Conditions

2. Fig 1
Adjustment of the acquisition dot plots for analysis of the neutrophil respiratory burst due to the green rhodamine fluorescence. (A) Forward scatter (FSC) versus sideward scatter signals (SSC) were depicted as acquired on the flow cytometer. Neutrophils were localized and gated based on their high SSC signals. Only neutrophils from this region were used in the next dot plot. (B) SSC versus red propidium iodide fluorescence (photomultiplier FL3) for gating of necrotic neutrophils (‘dead cells’) based on their strong red fluorescence. Cells from this gate were excluded in the following dot plots. (C and D) SSC versus green rhodamine fluorescence (photomultiplier FL1) for gating of rhodamine-positive cells using the gate combination ‘granulocytes’ of dot plot A but not ‘dead cells’ of dot plot B. (C) is an example for a negative control without stimulation, whereas (D) depicts RB-positive PMNs after E. coli stimulation.
British Journal of Anaesthesia  , DOI: ( /bja/ )
Copyright © 2000 British Journal of Anaesthesia Terms and Conditions

3. Fig 2
Percentage of PMNs phagocytosing opsonized FITC-labelled E. coli (upper panel) and the phagocytic activity in single PMNs expressed by the mean green channel fluorescence (lower panel) in patients given propofol (•) or isoflurane (▿). *Significantly different from values before induction of anaesthesia (100%). There were no significant differences between propofol and isoflurane anaesthesia in either of the phagocytosis parameters tested. Data are expressed as mean – sd.
British Journal of Anaesthesia  , DOI: ( /bja/ )
Copyright © 2000 British Journal of Anaesthesia Terms and Conditions

4. Fig 3
Percentage of PMNs producing superoxide anions during the respiratory burst after stimulation with E. coli (upper panel) and TNF-α/FMLP (lower panel) in patients given propofol (•) or isoflurane (▿). *Significantly different from values before induction of anaesthesia (100%). †Significant difference between propofol and isoflurane. Data are expressed as mean – sd.
British Journal of Anaesthesia  , DOI: ( /bja/ )
Copyright © 2000 British Journal of Anaesthesia Terms and Conditions