1. Detection and isolation of MKWV
Detection and isolation of MKWV. (a and b) Ticks (Ixodes persulcatus) collected in Mukawa, Hokkaido, Japan (a), were genetically screened for tick-borne phleboviruses (TBPVs) by using the pan-TBPV RT-PCR (b).
Detection and isolation of MKWV. (a and b) Ticks (Ixodes persulcatus) collected in Mukawa, Hokkaido, Japan (a), were genetically screened for tick-borne phleboviruses (TBPVs) by using the pan-TBPV RT-PCR (b). Numbers at top are sample number designations. (c) The nucleotide sequences of amplified DNA fragments were phylogenetically analyzed with those of other phleboviruses, and all MKWV fragments clustered together in the independent branch. (d) Virions of the MKWV isolate MKW73, harvested from the ISE6 tick embryonic cell supernatant, were morphologically analyzed using scanning electron microscopy. Bar, 100 nm. SFTSV, severe fever with thrombocytopenia syndrome virus; RVFV, Rift Valley fever virus.
Keita Matsuno et al. mSphere 2018; doi: /mSphere