1. Higher Levels of Steroidogenic Acute Regulatory Protein and Type I 3β-Hydroxysteroid Dehydrogenase in the Scalp of Men with Androgenetic Alopecia 
WenChieh Chen, Shaw-Jenq Tsai, Chen-Yu Liao, Ren-Yeu Tsai, Yi-Ju Chen, Bi-Jen Pan, Chiu-Ling Hung, Christos C. Zouboulis 
Journal of Investigative Dermatology 
Volume 126, Issue 10, Pages (October 2006)
DOI: /sj.jid
Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Detection and quantification of mRNA. After preparation and construction of the native and competitive plasmids for in vitro transcription of respective RNAs, the standard curves of quantitative competitive RT-PCR for each measured genes including (a) StAR and (b) type I 3β-HSD1 were generated by analyzing the intensity of bands derived from ethidium bromide-stained PCR products. A 2-fold serial dilution of native RNA was reverse transcribed and PCR amplified in the presence of 1am competitor. The band intensity was quantified by AlphaImager computer software. Representative gel picture showing PCR products amplified from paired specimens taken from the bald fronto-parietal (B) and hairy occipital (H) scalps of men with AGA during hair transplantation. After calibration by the expressional intensity of glyceraldehyde 3-phosphate dehydrogenase (GAPDH), a B/H ratio for the measured mRNA levels was obtained from each individual subject regarding (c) StAR and (d) 3β-HSD1, respectively. Mean value of B/H ratio was calculated for StAR and 3β-HSD1 from 48 and 46 patients, respectively. As compared with the enzyme expression in the occipital scalp (H, taken as 1), significantly higher levels of StAR and 3β-HSD1 were found in the bald fronto-parietal scalp (P<0.05). NC, negative control by omitting reverse transcriptase during the RT procedure.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Immunodetection of StAR in vitro and in vivo. A specific rabbit polyclonal antibody raised against amino acids 1–285 representing full-length StAR of human origin (Santa Cruz, Santa Cruz, CA) was used and diluted to 1:100 and 1:50 in diluent (DAKO, Glostrup, Denmark) for cultured cells and for skin derived from vertical scalp of a 24-year-old man without AGA, respectively. StAR was detected mainly in (a) the perinuclear cytoplasm of the cultured SZ95 sebocytes, (b) basal layer of the sebaceous glands, (c) epidermal keratinocytes, (d) outer root sheath of hair follicles (asterisk), (e) vascular tissues (arrowhead), and (f) eccrine sweat ducts (arrow) but not eccrine sweat glands. Note the stronger expression of StAR protein in the small undifferentiated sebocytes (a and b). Bar=20μm.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions