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Volume 64, Issue 3, Pages (September 2003)

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1 Volume 64, Issue 3, Pages 906-913 (September 2003)
Mast cell infiltration and chemokine expression in progressive renal disease1  Susan E. Jones, Darren J. Kelly, Alison J. Cox, Yuan Zhang, Renae M. Gow, Richard E. Gilbert  Kidney International  Volume 64, Issue 3, Pages (September 2003) DOI: /j x Copyright © 2003 International Society of Nephrology Terms and Conditions

2 Figure 1 Mast cells in subtotal nephrectomy. ACEI is angiotensin-converting enzyme inhibitor. Kidney International  , DOI: ( /j x) Copyright © 2003 International Society of Nephrology Terms and Conditions

3 Figure 2 Photomicrographs of mast cells within the kidney of subtotally nephrectomized rats. Chymase-positive mast cells (A) (arrows) localized to the cortical interstitium (magnification ×280). Serial sections showing an interstitial mast cell in close proximity to a vessel (*) demonstrating toluidine blue metachromasia (B), and positive immunostaining for tryptase (C) and transforming growth factor-β (TGF-β) (D) (magnification ×1020). Kidney International  , DOI: ( /j x) Copyright © 2003 International Society of Nephrology Terms and Conditions

4 Figure 3 Serial sections showing (A) toluidine blue–stained mast cells (arrows) and (B) ED-1 immunopositive macrophages (arrows) localized to the interstitium and adjacent to a tubule (*) (magnification ×520). Kidney International  , DOI: ( /j x) Copyright © 2003 International Society of Nephrology Terms and Conditions

5 Figure 4 Mast cell chemokines. Graphs showing gene expression of (A) stem cell factor (SCF), (B) interleukin-8 (IL-8/gro-α) and (C) transforming growth factor-β1 (TGF-β1) relative to the housekeeping gene 18S, as assessed by real-time polymerase chain reaction (PCR). Results are expressed in arbitrary units relative to sham, assigned a value of 1. *P < 0.01 vs. sham; †P < 0.01 vs. 12 weeks. Kidney International  , DOI: ( /j x) Copyright © 2003 International Society of Nephrology Terms and Conditions

6 Figure 4 Mast cell chemokines. Graphs showing gene expression of (A) stem cell factor (SCF), (B) interleukin-8 (IL-8/gro-α) and (C) transforming growth factor-β1 (TGF-β1) relative to the housekeeping gene 18S, as assessed by real-time polymerase chain reaction (PCR). Results are expressed in arbitrary units relative to sham, assigned a value of 1. *P < 0.01 vs. sham; †P < 0.01 vs. 12 weeks. Kidney International  , DOI: ( /j x) Copyright © 2003 International Society of Nephrology Terms and Conditions

7 Figure 5 Photomicrograph showing stem cell factor (SCF)–stained tubular epithelium (brown) in an area of tubulointerstitial injury, adjacent to a glomerulus (*) from a 5/6 nephrectomized rat (magnification ×280). Kidney International  , DOI: ( /j x) Copyright © 2003 International Society of Nephrology Terms and Conditions


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