1. Epidermal Stem Cells do not Communicate Through Gap Junctions
Maja Matic, W. Howard Evans, Peter R. Brink, Marcia Simon 
Journal of Investigative Dermatology 
Volume 118, Issue 1, Pages (January 2002)
DOI: /j x x
Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Cx43 expression in human neonatal foreskin. Fluorescent micrographs of two different regions of the epidermis (A, B) and (C). Cx43 (FITC) expression (A, B). Yellow background in the basal cells in (A) is a “bleed through” the green filter of rhodamine-stained K19. The large arrow in (A) points to the cell that apparently lacks Cx43 expression. The small arrows point to the Cx43 puncta on the neighboring cells in the basal layer. Note the high density of Cx43 puncta on the cells in the suprabasal layers (B). Double fluorescence, K19 (rhodamine) and Cx43 (FITC) expression (C). The arrows in (C) point to the cells with no apparent Cx43 expression. Scale bar: 4.8 µm.
Journal of Investigative Dermatology  , DOI: ( /j x x)
Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Cx43 expression in the bulge region of the human hair follicle. Fluorescent micrographs. Low magnification view (A, B). The rectangle marks the region viewed under high magnification (C, D). Cx43 (FITC) expression (A, C). K19 (rhodamine) expression (B). Double fluorescence, Cx43 (FITC), K19 (rhodamine) expression in the bulge (D). Arrows point to the cells in the basal layer of the outer root sheath with no apparent expression of Cx43 (C). Inner root sheath in (C) is overexposed in order for Cx43-free cells to be visible due to “bleed through” of rhodamine-stained K19. Arrowheads in (D) point to the Cx43 puncta in cells adjacent to those that are Cx43 negative. s, sebaceous gland. Scale bar: (A, B) 96 µm; (C, D) 12 µm.
Journal of Investigative Dermatology  , DOI: ( /j x x)
Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Flow cytometry analysis. Dot plot in arbitrary units on a linear scale showing FSC and SSC (A). Each dot represents the FSC and SSC values for a single cell. An oval gate is used to exclude cell debris and select cells for dual fluorescence analysis. Dot plot showing fluorescence intensity of keratinocytes double labeled with antibody against Cx43 (R-PE), y axis, and antibody against K14 (FITC), x axis, measured in arbitrary units on a log scale (B). Quadrants are established using isotype controls, and single color positive controls. Suprabasal cells (Cx43+, K14-) are located in the upper left quadrant, R4. Presumptive stem cells (Cx43-, K14+) are located in the lower right quadrant, R2, and the rest of the basal cells (Cx43+, K14+) are located in the upper right quadrant, R3. Cells with nonspecific binding are located in the lower left quadrant, R0, and probably present a nonkeratinocyte population. Dot plots showing FSC and SSC values (C-E) for presumptive stem cells (C); basal cells excluding presumptive stem cells (D); and suprabasal cells (E). Data are representative of five separate experiments.
Journal of Investigative Dermatology  , DOI: ( /j x x)
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5. Figure 4
Microinjections of fluorescent dyes into neonatal foreskin epithelium. Junctional permeability was assayed by microinjection of Lucifer yellow and carboxyfluorescein into single cells. An example of the absence of dye coupling in basal cells (A). Photomicrograph showing transfer of Lucifer yellow and carboxyfluorescein to a neighboring cell in the basal layer (B). The dye-containing cells appear diffuse, as the fluorescence was observed through multiple cell layers. Scanning confocal micrographs of dye transfer upon injection of a suprabasal cell (C-E). Optical sectioning of the epithelial sheath was performed at 4 µm. Three optical sections are shown. Extensive coupling occurs into cells of the same layer and into cells of other layers. Arrows point to the microinjected cells. The micrographs represent typical results following dye injections. Light (F) and fluorescence (G) micrographs of a cross-section of the epithelial sheath showing a basal cell microinjected with Lucifer yellow. Note that no dye was transferred to neighboring cells. Scale bar: (A, B) approximately 4 µm; (C-E) 20 µm; (F, G) 12 µm.
Journal of Investigative Dermatology  , DOI: ( /j x x)
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6. Figure 5
LRC and Cx43 expression. Sections of the bulge area of vibrissal (A, B), and pelage follicles (C, D). (A, C) Bright field micrographs; (B, D), fluorescence micrographs. (B′) Diagram of (A) and (B). The focal plane was adjusted to visualize either Cx43 (B, D), or [3H]thymidine (A, C). The section in (B) was photographed through a green filter to allow some background autofluorescence to be visible for orientation purposes. Arrows point to the cells that express Cx43. Note that LRC do not express Cx43. Scale bar: 12 µm.
Journal of Investigative Dermatology  , DOI: ( /j x x)
Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions