1. E7-less STING isoforms are proviral replication factors.
E7-less STING isoforms are proviral replication factors. (A) PMA-differentiated THP1 macrophages were transfected with E7-less hSTING isoforms–specific siRNA (236) or irrelevant siRNA control (SC). siRNA knockdown efficiency was tested by quantitative RT-PCR (qRT-PCR) using wt or E7-less hSTING isoforms–specific primers. (B) IFN-β expression 16 h posttransfection of poly(dA:dT) in scramble or E7-less hSTING isoforms knock down THP1 macrophages determined by qRT-PCR. (C) IFN-β expression in scramble or E7-less hSTING isoforms knock down THP1 macrophages postinfection with HSV-1 (MOI: 10) at the indicated time points. (D) HSV-1 titers determined by plaque assay in scramble or E7-less hSTING isoforms knock down THP1 macrophages 24 h postinfection at an MOI of 0.1. (E) Replication titers of VSV-GFP determined by TCID50 in scramble or E7-less hSTING isoforms knock down THP1 macrophages 16 h postinfection with a starting MOI of (F–I) PMA-differentiated THP1 macrophages were infected at a MOI of 10 with HSV-1 (F) or VSV-GFP (H). Cells were harvested at the indicated time points postinfection, and wt and E7-less hSTING isoforms mRNA expression was determined by RT-PCR. Representation of relative wt/E7-less STING isoform expression after HSV-1 (G) or VSV-GFPI) infection (MOI: 10) at the indicated time points. **p < 0.1, ***p < 0.01.
Estefanía Rodríguez-García et al. ImmunoHorizons 2018;2:
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