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Comprehensive Genomic Profiling of Malignant Effusions in Patients with Metastatic Lung Adenocarcinoma  Soo-Ryum Yang, Chieh-Yu Lin, Henning Stehr, Steven.

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Presentation on theme: "Comprehensive Genomic Profiling of Malignant Effusions in Patients with Metastatic Lung Adenocarcinoma  Soo-Ryum Yang, Chieh-Yu Lin, Henning Stehr, Steven."— Presentation transcript:

1 Comprehensive Genomic Profiling of Malignant Effusions in Patients with Metastatic Lung Adenocarcinoma  Soo-Ryum Yang, Chieh-Yu Lin, Henning Stehr, Steven R. Long, Christina S. Kong, Gerald J. Berry, James L. Zehnder, Christian A. Kunder  The Journal of Molecular Diagnostics  Volume 20, Issue 2, Pages (March 2018) DOI: /j.jmoldx Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2 Figure 1 Patient samples and variant annotation. NGS, next-generation sequencing. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3 Figure 2 Correlation between variant allele frequency and tumor cellularity. A: Correlation between mean variant allele frequency and revised tumor cellularity. B: Correlation between mean variant allele frequency and original tumor cellularity. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

4 Figure 3 Mutational landscape of pathogenic or likely pathogenic alterations (PLPAs) across 27 patients. A: Co-mutation plot. B: Distribution of PLPAs in driver oncogenes. The bar on the right illustrates the number of different EGFR mutations detected in the EGFR-positive samples. Del, deletion; ins, insertion; VUS, variant of unknown significance. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

5 Figure 4 Comparison with additional molecular testing performed on preceding and subsequent samples. Effusion samples are shaded in gray. Patient 3 had two effusion samples that were both analyzed by next-generation sequencing (NGS). Data were last updated on January 1, FISH, fluorescence in situ hybridization; N/A, not available; SS/FA, SNaPshot/fragment analysis. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

6 Figure 5 Cytologic preparations for malignant effusions. A: Air-dried smear with Diff-Quick stain (Romanowsky stain) (70% estimated tumor cellularity). B: Liquid-based ThinPrep slide with Papanicolaou stain (70% estimated tumor cellularity). C: Formalin-fixed, paraffin-embedded cell block section with hematoxylin and eosin stain (50% estimated tumor cellularity). Detailed patient information is provided in Supplemental Table S3. Light smokers were with ≤5 lifetime pack-years. Significant smokers were those with >5 lifetime pack-years. Original magnification, ×10. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

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