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Cross-reactivity between Cupressus arizonica and Cupressus sempervirens pollen extracts  Bianca Barletta, BSc, Claudia Afferni, BSc, Raffaella Tinghino,

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Presentation on theme: "Cross-reactivity between Cupressus arizonica and Cupressus sempervirens pollen extracts  Bianca Barletta, BSc, Claudia Afferni, BSc, Raffaella Tinghino,"— Presentation transcript:

1 Cross-reactivity between Cupressus arizonica and Cupressus sempervirens pollen extracts 
Bianca Barletta, BSc, Claudia Afferni, BSc, Raffaella Tinghino, BSc, Adriano Mari, MD, Gabriella Di Felice, BSc, Carlo Pini, BSc  Journal of Allergy and Clinical Immunology  Volume 98, Issue 4, Pages (October 1996) DOI: /S (96)70129-X Copyright © 1996 Mosby, Inc. Terms and Conditions

2 FIG. 1 CaE (a, c, e) and CsE (b, d, f) pollen extracts after separation by SDS-PAGE in reducing conditions. Coomassie Brilliant Blue staining (a, b), immunoblotting developed with a pool of sera from subjects allergic to Cupressaceae (c, d), and immunoblotting developed with a pool of sera from normal subjects (e, f). Journal of Allergy and Clinical Immunology  , DOI: ( /S (96)70129-X) Copyright © 1996 Mosby, Inc. Terms and Conditions

3 FIG. 2 Immunoblotting inhibition pattern of anti-C. arizonica rabbit antiserum binding to CaE (A) and CsE (B), inhibited with CaE (lanes 1 to 8) or CsE (lanes 9 to 16); immunoblotting inhibition pattern of anti-C. sempervirens rabbit antiserum binding to CaE (C) and CsE (D), inhibited with CaE (lanes 1 to 8) or CsE (lanes 9 to 16). Amounts of inhibitors are indicated in Methods. Lane 17 shows the antiserum binding to blotted antigens in the absence of inhibitor. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96)70129-X) Copyright © 1996 Mosby, Inc. Terms and Conditions

4 FIG. 2 Immunoblotting inhibition pattern of anti-C. arizonica rabbit antiserum binding to CaE (A) and CsE (B), inhibited with CaE (lanes 1 to 8) or CsE (lanes 9 to 16); immunoblotting inhibition pattern of anti-C. sempervirens rabbit antiserum binding to CaE (C) and CsE (D), inhibited with CaE (lanes 1 to 8) or CsE (lanes 9 to 16). Amounts of inhibitors are indicated in Methods. Lane 17 shows the antiserum binding to blotted antigens in the absence of inhibitor. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96)70129-X) Copyright © 1996 Mosby, Inc. Terms and Conditions

5 FIG. 2 Immunoblotting inhibition pattern of anti-C. arizonica rabbit antiserum binding to CaE (A) and CsE (B), inhibited with CaE (lanes 1 to 8) or CsE (lanes 9 to 16); immunoblotting inhibition pattern of anti-C. sempervirens rabbit antiserum binding to CaE (C) and CsE (D), inhibited with CaE (lanes 1 to 8) or CsE (lanes 9 to 16). Amounts of inhibitors are indicated in Methods. Lane 17 shows the antiserum binding to blotted antigens in the absence of inhibitor. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96)70129-X) Copyright © 1996 Mosby, Inc. Terms and Conditions

6 FIG. 2 Immunoblotting inhibition pattern of anti-C. arizonica rabbit antiserum binding to CaE (A) and CsE (B), inhibited with CaE (lanes 1 to 8) or CsE (lanes 9 to 16); immunoblotting inhibition pattern of anti-C. sempervirens rabbit antiserum binding to CaE (C) and CsE (D), inhibited with CaE (lanes 1 to 8) or CsE (lanes 9 to 16). Amounts of inhibitors are indicated in Methods. Lane 17 shows the antiserum binding to blotted antigens in the absence of inhibitor. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96)70129-X) Copyright © 1996 Mosby, Inc. Terms and Conditions

7 FIG. 3 ELISA inhibition of anti-C. arizonica rabbit antiserum tested against CaE (A) and CsE (B) and of anti-C. sempervirens rabbit antiserum tested against CaE (C) and CsE (D). Inhibitors were CaE (filled circles) and CsE (open circles). Journal of Allergy and Clinical Immunology  , DOI: ( /S (96)70129-X) Copyright © 1996 Mosby, Inc. Terms and Conditions

8 FIG. 3 ELISA inhibition of anti-C. arizonica rabbit antiserum tested against CaE (A) and CsE (B) and of anti-C. sempervirens rabbit antiserum tested against CaE (C) and CsE (D). Inhibitors were CaE (filled circles) and CsE (open circles). Journal of Allergy and Clinical Immunology  , DOI: ( /S (96)70129-X) Copyright © 1996 Mosby, Inc. Terms and Conditions

9 FIG. 3 ELISA inhibition of anti-C. arizonica rabbit antiserum tested against CaE (A) and CsE (B) and of anti-C. sempervirens rabbit antiserum tested against CaE (C) and CsE (D). Inhibitors were CaE (filled circles) and CsE (open circles). Journal of Allergy and Clinical Immunology  , DOI: ( /S (96)70129-X) Copyright © 1996 Mosby, Inc. Terms and Conditions

10 FIG. 3 ELISA inhibition of anti-C. arizonica rabbit antiserum tested against CaE (A) and CsE (B) and of anti-C. sempervirens rabbit antiserum tested against CaE (C) and CsE (D). Inhibitors were CaE (filled circles) and CsE (open circles). Journal of Allergy and Clinical Immunology  , DOI: ( /S (96)70129-X) Copyright © 1996 Mosby, Inc. Terms and Conditions

11 FIG. 4 ELISA inhibition of pooled human sera from patients allergic to Cupressaceae tested against CaE (A) and CsE (B). Inhibitors were CaE (filled circles) and CsE (open circles). Journal of Allergy and Clinical Immunology  , DOI: ( /S (96)70129-X) Copyright © 1996 Mosby, Inc. Terms and Conditions

12 FIG. 4 ELISA inhibition of pooled human sera from patients allergic to Cupressaceae tested against CaE (A) and CsE (B). Inhibitors were CaE (filled circles) and CsE (open circles). Journal of Allergy and Clinical Immunology  , DOI: ( /S (96)70129-X) Copyright © 1996 Mosby, Inc. Terms and Conditions

13 FIG. 5 Immunoblotting inhibition pattern of IgE binding to components of CaE (A) and CsE (B) inhibited with CaE (lanes 1 to 8) or CsE (lanes 9 to 16). Amounts of inhibitors are indicated in Methods. Lane 17 shows IgE binding to blotted antigens in the absence of inhibitor. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96)70129-X) Copyright © 1996 Mosby, Inc. Terms and Conditions

14 FIG. 5 Immunoblotting inhibition pattern of IgE binding to components of CaE (A) and CsE (B) inhibited with CaE (lanes 1 to 8) or CsE (lanes 9 to 16). Amounts of inhibitors are indicated in Methods. Lane 17 shows IgE binding to blotted antigens in the absence of inhibitor. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96)70129-X) Copyright © 1996 Mosby, Inc. Terms and Conditions

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