1. Volume 8, Issue 3, Pages 167-174 (June 2002)
Change in free radical-related substances in plasma following ischemia-reperfusion in rat liver 
Atsuko Iwamoto, Toru Egashira, Fusako Takayama, Yasumitsu Yamanaka, Takayuki Noguchi 
Pathophysiology 
Volume 8, Issue 3, Pages (June 2002)
DOI: /S (02)00010-X

2. Fig. 1
Luminol-enhanced CL from rat blood stimulated by PMA. The blood samples were collected after 60 min of ischemia and at various reperfusion times. CL: chemiluminescence, PMA: phorbol myristate acetate. Each value represents the mean±S.D. of five rats. The experimental values were significantly different from those of the pre-ischemia controls at *, P<0.05.
Pathophysiology 2002 8, DOI: ( /S (02)00010-X)

3. Fig. 2
Plasma lipid peroxide levels in rats treated with ischemia-reperfusion. Lipid peroxidation was estimated by t-BuOOH-initiated CL. Each value represents the mean±S.D. of five rats. The experimental values were significantly different from those of the pre-ischemia controls at *, P<0.01.
Pathophysiology 2002 8, DOI: ( /S (02)00010-X)

4. Fig. 3
Concentrations of plasma NOx in rats treated with ischemia-reperfusion. The blood samples were collected after 60 min of ischemia and at various reperfusion times. Each value represents the mean±S.D. of five rats. The experimental values were significantly different from those of the pre-ischemia controls at *, P<0.05.
Pathophysiology 2002 8, DOI: ( /S (02)00010-X)

5. Fig. 4
Changes in SOD-like activity in the plasma following ischemia-reperfusion in rat liver (short reperfusion time). The blood samples were collected after 60 min of ischemia and at 5, 10, 20, 40, 60, 120 or 180 min of reperfusion. SOD-like activity is expressed as U/ml plasma. –○–, Cu,Zn-SOD-like activity; –●–, Mn-SOD-like activity. Each value represents the mean±S.D., of five rats. The experimental values were significantly different from those of the pre-ischemia controls at *, P<0.05.
Pathophysiology 2002 8, DOI: ( /S (02)00010-X)

6. Fig. 5
Changes in SOD-like activity in the plasma following ischemia-reperfusion in rat liver (long reperfusion time). The blood samples were collected after 60 min of ischemia and at 0.5, 1, 2, 6, 10, 20, 24 or 48 h of reperfusion. SOD-like activity is expressed as U/ml plasma. –○–, Cu,Zn-SOD-like activity; –●–, Mn-SOD-like activity. Each value represents the mean±S.D. of five rats. The experimental values were significantly different from those of the pre-ischemia controls at *, P<0.05.
Pathophysiology 2002 8, DOI: ( /S (02)00010-X)

7. Fig. 6
Detection of Cu,Zn-SOD and Mn-SOD in plasma by Western blot analysis. Upper panel, the blood samples were collected after 60 min of ischemia and at various reperfusion times (short reperfusion time). Lane p: standard Mn-SOD and Cu,Zn-SOD, lane 1, pre-ischemia; lane 2, ischemia; lane 3, 5 min; lane 4, 10 min; lane 5, 20 min; lane 6, 40 min; lane 7, 80 min; lane 8, 120 min; lane 9, 180 min. Bottom panel, the blood samples were collected after 60 min of ischemia and at various reperfusion times (long reperfusion time). Lane p, standard Mn-SOD and Cu,Zn-SOD; lane 1, pre-ischemia; lane 2, ischemia; lane 3, 0.5 h; lane 4, 1 h; lane 5, 2 h; lane 6, 6 h; lane 7, 10 h; lane 8, 24 h; lane 9, 48 h.
Pathophysiology 2002 8, DOI: ( /S (02)00010-X)