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Distinction of Desmoplastic Melanoma from Non-Desmoplastic Melanoma by Gene Expression Profiling
Klaus J. Busam, Hui Zhao, Daniel G. Coit, Derya Kucukgol, Achim A. Jungbluth, Jennifer Nobrega, Agnes Viale Journal of Investigative Dermatology Volume 124, Issue 2, Pages (February 2005) DOI: /j X x Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions
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Figure 1 Histologic appearance of desmoplastic (DM) and conventional epithelioid, i.e. non-desmoplastic melanoma (NDM). In DM (A), atypical fusiform melanocytes are dispersed in a collagenous stroma. In NDM (B), epithelioid melanocytes are arranged in nested cellular aggregates with little or no intratumoral fibrosis. Journal of Investigative Dermatology , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions
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Figure 2 Hierarchical clustering of 629 genes separates desmoplastic melanoma (DM) from conventional non-desmoplastic melanoma (NDM). Cases 1–5 represent melanomas with desmoplasia (1–4 are typical DMs, case 5 is a melanoma with only partial desmoplasia tumors, 6–10 are melanomas without demoplasia). Journal of Investigative Dermatology , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions
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Figure 3 Immunohistochemical expression of clusterin (tissue microarray analysis). (A) Hematoxylin and eosin (H&E)-stained section of desmoplastic melanoma (DM). (B) DM is immunopositive for clusterin. (C) H&E stain of non-desomplastic melanoma (NDM). (D) NDM is immunonegative for clusterin. Journal of Investigative Dermatology , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions
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