Presentation is loading. Please wait.

Presentation is loading. Please wait.

Notch signaling confers antigen-presenting cell functions on mast cells  Nobuhiro Nakano, PhD, Chiharu Nishiyama, PhD, Hideo Yagita, PhD, Akemi Koyanagi,

Published byAriel Hilary Turner Modified over 5 years ago

Similar presentations

Presentation on theme: "Notch signaling confers antigen-presenting cell functions on mast cells  Nobuhiro Nakano, PhD, Chiharu Nishiyama, PhD, Hideo Yagita, PhD, Akemi Koyanagi,"— Presentation transcript:

1 Notch signaling confers antigen-presenting cell functions on mast cells 
Nobuhiro Nakano, PhD, Chiharu Nishiyama, PhD, Hideo Yagita, PhD, Akemi Koyanagi, PhD, Hisaya Akiba, PhD, Shigeru Chiba, MD, PhD, Hideoki Ogawa, MD, PhD, Ko Okumura, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 123, Issue 1, Pages e1 (January 2009) DOI: /j.jaci Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Cell surface expression of Notch receptors on mast cells. The expressions of Notch 1, 2, 3, or 4 (bold lines) on c-Kit+FcɛRIα+ BMMCs cultured for 2, 3, or 4 weeks (A) or fresh c-Kit+FcɛRIα+ PMCs (B) were analyzed by flow cytometry. Thin lines represent isotype-matched control. A representative of 3 repeated experiments with similar results is shown. Journal of Allergy and Clinical Immunology  , e1DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Notch signaling induces MHC-II expression on mast cell surface. BMMCs (A) or PMCs (B) were cocultured with the indicated CHO–Notch ligand—expressing cell lines or CHO control cells in the presence or absence of DAPT. Cont., Control. Dot blots show the expressions of MHC-II (I-A) and c-Kit on FcɛRIα+ cells. Data in the graphs show the ratio of I-Ad+ cells in c-Kit+FcɛRIα+ cells, and are indicated as means ± SDs of quadruplicate samples. ∗P < .05; ∗∗P < .005, as determined by the Student t test. Journal of Allergy and Clinical Immunology  , e1DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Notch signaling upregulates OX40L expression level on mast cell surface. BMMCs (A) or PMCs (B) were cocultured with the indicated CHO–Notch ligand—expressing cell lines or CHO control cells in the presence or absence of DAPT. The expression of OX40L (bold lines) on c-Kit+FcɛRIα+ cells was analyzed by flow cytometry. Thin lines represent isotype-matched control. A representative of 3 repeated experiments with similar results is shown. Journal of Allergy and Clinical Immunology  , e1DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Effects of Dll1/Notch signaling on FcɛRI-mediated mast cell responses. A, FcɛRIα expression (bold lines) on control-BMMCs or Dll1-BMMCs. B, The expressions of MHC-II and OX40L on control-BMMCs or Dll1-BMMCs in response to FcɛRI cross-linking. Dot blots show the expressions of MHC-II (I-A) and c-Kit on FcɛRIα+ cells. Histograms show OX40L expression (bold lines) on c-Kit+FcɛRIα+ cells. Thin lines represent isotype-matched control. A representative of 3 repeated experiments with similar results is shown. C, β-Hexosaminidase activity and production of eicosanoids (prostaglandin D2, LTB4, and cysLTs) and cytokines in control-BMMCs or Dll1-BMMCs in response to FcɛRI cross-linking. Data are indicated as means ± SDs of triplicate samples. ∗P < .05; ∗∗P < .005; N.S., not significant, as determined by the Student t test. Journal of Allergy and Clinical Immunology  , e1DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 CD4+ T-cell activation mediated by Dll1-BMMCs. [3H]Thymidine incorporation into the CD4+ T cells from DO11.10 mice cocultured together with BMMCs or BMDCs (A) or separated from them by a Transwell membrane (B) in the presence or absence of ovalbumin (OVA) peptide. Data are indicated as means ± SDs of triplicate samples. †P < .05 vs T cells alone in the presence of OVA peptide; ∗P < .05 vs corresponding values for T cells plus control-BMMCs. MC, Mast cell; DC, dendritic cell. C, IL-2 level in the supernatants when CD4+ T cells from DO11.10 mice were direct cocultured with BMMCs or BMDCs at 48 hours in the presence of OVA peptide. Data are indicated as means ± SDs of triplicate samples. D, [3H]Thymidine incorporation into the CD4+ T cells from DO11.10 mice direct cocultured with BMMCs or BMDCs in the presence of OVA peptide and anti-OX40L neutralizing mAb at indicated concentrations. Data are indicated as means ± SDs of triplicate samples. ∗P < .05; ∗∗P < .005, significantly different as determined by the Student t test compared with the corresponding control. Journal of Allergy and Clinical Immunology  , e1DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig 6 Dll1-BMMCs enhance IL-4, IL-10, and IL-13 production and suppress IFN-γ production by CD4+ T cells. Purified CD4+ T cells from DO11.10 mice were direct cocultured with BMMCs or BMDCs in the presence of ovalbumin peptide. After 4 days of coculture, the cocultured cells were restimulated with anti-CD3 mAb and anti-CD28 mAb for 24 hours, and then the cytokine concentrations in the supernatants were measured. Data are indicated as means ± SDs of triplicate samples. †P < .05 vs T cells alone; ∗P < .05 vs T cells plus control-BMMCs. Journal of Allergy and Clinical Immunology  , e1DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Notch signaling confers antigen-presenting cell functions on mast cells  Nobuhiro Nakano, PhD, Chiharu Nishiyama, PhD, Hideo Yagita, PhD, Akemi Koyanagi,"

Similar presentations

Alternation of circadian clock modulates forkhead box protein-3 gene transcription in CD4+ T cells in the intestine  Gui Yang, MD, Hong Zhang, PhD, Yi.

Alternation of circadian clock modulates forkhead box protein-3 gene transcription in CD4+ T cells in the intestine  Gui Yang, MD, Hong Zhang, PhD, Yi.
Identification of drug- and drug-metabolite immune responses originating from both naive and memory T cells  Andrew Gibson, MRes, Lee Faulkner, PhD, Sally.

Identification of drug- and drug-metabolite immune responses originating from both naive and memory T cells  Andrew Gibson, MRes, Lee Faulkner, PhD, Sally.
Early suppression of basophil activation during allergen-specific immunotherapy by histamine receptor 2  Natalija Novak, MD, Nihal Mete, MD, Caroline.

Early suppression of basophil activation during allergen-specific immunotherapy by histamine receptor 2  Natalija Novak, MD, Nihal Mete, MD, Caroline.
Flow cytometry imaging identifies rare TH2 cells expressing thymic stromal lymphopoietin receptor in a “proallergic” milieu  Amanda J. Reefer, MS, Kathryn.

Flow cytometry imaging identifies rare TH2 cells expressing thymic stromal lymphopoietin receptor in a “proallergic” milieu  Amanda J. Reefer, MS, Kathryn.
IgE-mediated systemic anaphylaxis and impaired tolerance to food antigens in mice with enhanced IL-4 receptor signaling  Clinton B. Mathias, PhD, Suejy.

IgE-mediated systemic anaphylaxis and impaired tolerance to food antigens in mice with enhanced IL-4 receptor signaling  Clinton B. Mathias, PhD, Suejy.
Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 

Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 
Mechanical injury polarizes skin dendritic cells to elicit a TH2 response by inducing cutaneous thymic stromal lymphopoietin expression  Michiko K. Oyoshi,

Mechanical injury polarizes skin dendritic cells to elicit a TH2 response by inducing cutaneous thymic stromal lymphopoietin expression  Michiko K. Oyoshi,
Alternation of circadian clock modulates forkhead box protein-3 gene transcription in CD4+ T cells in the intestine  Gui Yang, MD, Hong Zhang, PhD, Yi.

Alternation of circadian clock modulates forkhead box protein-3 gene transcription in CD4+ T cells in the intestine  Gui Yang, MD, Hong Zhang, PhD, Yi.
IgE cross-linking impairs monocyte antiviral responses and inhibits influenza-driven TH1 differentiation  Regina K. Rowe, MD, PhD, David M. Pyle, MD,

IgE cross-linking impairs monocyte antiviral responses and inhibits influenza-driven TH1 differentiation  Regina K. Rowe, MD, PhD, David M. Pyle, MD,
Reduced IFN-γ receptor expression and attenuated IFN-γ response by dendritic cells in patients with atopic dermatitis  Eva Gros, MSc, Susanne Petzold,

Reduced IFN-γ receptor expression and attenuated IFN-γ response by dendritic cells in patients with atopic dermatitis  Eva Gros, MSc, Susanne Petzold,
IL-25 and CD4+ TH2 cells enhance type 2 innate lymphoid cell–derived IL-13 production, which promotes IgE-mediated experimental food allergy  Jee-Boong.

IL-25 and CD4+ TH2 cells enhance type 2 innate lymphoid cell–derived IL-13 production, which promotes IgE-mediated experimental food allergy  Jee-Boong.
Virus-specific IgE enhances airway responsiveness on reinfection with respiratory syncytial virus in newborn mice  Azzeddine Dakhama, PhD, Young-Mok Lee,

Virus-specific IgE enhances airway responsiveness on reinfection with respiratory syncytial virus in newborn mice  Azzeddine Dakhama, PhD, Young-Mok Lee,
Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.

Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.
Toll-like receptor 7–induced naive human B-cell differentiation and immunoglobulin production  Mark C. Glaum, MD, PhD, Shilpi Narula, MD, Decheng Song,

Toll-like receptor 7–induced naive human B-cell differentiation and immunoglobulin production  Mark C. Glaum, MD, PhD, Shilpi Narula, MD, Decheng Song,
Allergic sensitization can be induced via multiple physiologic routes in an adjuvant- dependent manner  David Dunkin, MD, M. Cecilia Berin, PhD, Lloyd.

Allergic sensitization can be induced via multiple physiologic routes in an adjuvant- dependent manner  David Dunkin, MD, M. Cecilia Berin, PhD, Lloyd.
Oral immunotherapy induces IgG antibodies that act through FcγRIIb to suppress IgE- mediated hypersensitivity  Oliver T. Burton, PhD, Stephanie L. Logsdon,

Oral immunotherapy induces IgG antibodies that act through FcγRIIb to suppress IgE- mediated hypersensitivity  Oliver T. Burton, PhD, Stephanie L. Logsdon,
Increased expression of nuclear factor of activated T cells 1 drives IL-9–mediated allergic asthma  Sonja Koch, PhD, Anna Graser, PhD, Hooman Mirzakhani,

Increased expression of nuclear factor of activated T cells 1 drives IL-9–mediated allergic asthma  Sonja Koch, PhD, Anna Graser, PhD, Hooman Mirzakhani,
Ramses Ilarraza, PhD, Yingqi Wu, Christopher D

Ramses Ilarraza, PhD, Yingqi Wu, Christopher D
Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro  Pierre Pochard, PhD, Brian Vickery, MD, M. Cecilia.

Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro  Pierre Pochard, PhD, Brian Vickery, MD, M. Cecilia.
Autocrine hemokinin-1 functions as an endogenous adjuvant for IgE-mediated mast cell inflammatory responses  Tina L. Sumpter, PhD, Chin H. Ho, MD, Anna.

Autocrine hemokinin-1 functions as an endogenous adjuvant for IgE-mediated mast cell inflammatory responses  Tina L. Sumpter, PhD, Chin H. Ho, MD, Anna.

Similar presentations

Ads by Google