1. The Microscope
Ms. Duncan
Biology 1

2. Properties of Microscopes
Device used to magnify images of structures too small to see with the naked eye
Magnification: increases the size of the image
Resolution: shows clarity and detail of image
Types of Microscopes: Light Microscope & Electron Microscope

3. Light Microscope Magnifies using visible light rays
Simple microscopes: use one lens
Compound Light microscopes: use several lenses

4. Light Microscope
Photo: Courtesy of

5. Sample Images
Photos: courtesy of

6. Parts of the Compound Microscope
Ocular Lens: magnifies image at eyepiece (10X)
Eyepiece: where you look into the microscope
Objective Lens: the magnifying lens
High Power: 40X
Medium Power: 10X
Low Power: 4X

7. Parts of the Compound Microscope
Nosepiece: turns objectives
Stage: where slide sits upon
Diaphragm: controls amount of light allowed in view
Fine/Coarse Adjustment: adjusts sharpness of image
Arm: holds eyepiece and allows you to carry
Illuminator: source of light

9. Using the Microscope Total Magnification:
Magnification of objective lens x magnification of eyepiece
Example: The eyepiece will always be 10X. If you are using the low power objective (10X) then your total magnification is ????
10X x 10X = 100X

10. Using the Microscope
Focusing
Where to start
How

11. Microscope Development
Hans and Zacharis Jannsen: constructed the first microscope
Anton van Leeuwenhoek: “Father of Microscopy” *first person to see tiny living organisms living in a drop of water
First to study: Romans & Egyptians in 100 AD

12. Jannsen
Compliments of “The Gemmary.”

13. Leeuwenhoek
Photos: courtesy of

14. Hooke
Photos: courtesy of

15. Electron Microscope
Made in Europe during the 1950s, use beams of electrons instead of light.
Allows for more precise images

16. Electron Microscope Two types TEM: Transmission Electron Microscope
Shines a beam of electrons thru thin specimen
SEM: Scanning Electron Microscope
Run pencil-like beam of electrons back and forth across surface of specimen
Produce realistic and dramatic 3D images

17. TEM: transmission electron microscope Shines a beam of electrons thru thin specimen
Photo: courtesy of

18. Sample Images Electron Diffraction from a diamond
Diseased kidney tissue
Gold particle
Photos: Courtesy of

19. SEM: Scanning Electron Microscope Run pencil-like beam of electrons back and forth across surface of specimen Produce realistic and dramatic 3D images

20. Sample Images
Clam Gills
Kosher Salt
Black Widow Claw