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Chronic fatigue syndrome: Identification of distinct subgroups on the basis of allergy and psychologic variables  Larry Borish, MDa, Karen Schmaling,

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Presentation on theme: "Chronic fatigue syndrome: Identification of distinct subgroups on the basis of allergy and psychologic variables  Larry Borish, MDa, Karen Schmaling,"— Presentation transcript:

1 Chronic fatigue syndrome: Identification of distinct subgroups on the basis of allergy and psychologic variables  Larry Borish, MDa, Karen Schmaling, PhDc, Jeannie D. DiClementi, PsyDa, Joanne Streib, BAb, Julie Negri, BSa, James F. Jones, MDb  Journal of Allergy and Clinical Immunology  Volume 102, Issue 2, Pages (August 1998) DOI: /S (98) Copyright © 1998 Mosby, Inc. Terms and Conditions

2 Fig. 1 IFN-α and TNF-α ELISA. PBMCs were obtained from normal subjects and individuals with CFS. Cells were lysed by means of repeat freeze-thawing, and lysate was assayed for IFN-α and TNF-α by using commercial ELISA kits. Data were normalized to number of PBMCs. Individual data points, as well as means and SEM, are shown. Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions

3 Fig. 2 IL-10 ELISA. IL-10 concentrations were determined by ELISA from normal subjects and subjects with CFS. Individual data points, as well as means and SEM, are shown. Data are expressed as mean picograms of IL-10 per 10 7 PBMCs. Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions

4 Fig. 3 Evaluation of IFN-α, TNF-α, and IL-10 at baseline and during self-reported exacerbations of CFS symptoms. Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions

5 Fig. 4 Evaluation of IFN-α, TNF-α, and IL-10 at baseline and during seasonal exposure to allergens in allergic individuals. Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions

6 Fig. 5 RT-PCR. PBMCs were obtained from subjects as described, and total RNA was isolated. After generating cDNA, preliminary PCR studies were performed to determine the optimal number of cycles to visualize samples in the logarithmic phase of expansion. PCR was subsequently performed for TNF-α and β-actin, and these PCR products from each individual were pooled and electrophoresed. Samples were electrophoretically transferred to nylon paper, hybridized to 32P-labeled nested probes for TNF-α and β-actin, and autoradiography was performed. Representative data are displayed. Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions

7 Fig. 6 RT-PCR was performed for TNF-α. After hybridization to a 32P-labeled nested probe and autoradiography, densitometric analysis was performed. Data were normalized to results observed with β-actin. Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions


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