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Robert J. Rizzo, MD, Walter J. McCarthy, MD, Saryu N

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1 Collagen types and matrix protein content in human abdominal aortic aneurysms 
Robert J. Rizzo, MD, Walter J. McCarthy, MD, Saryu N. Dixit, PhD a, Michael P. Lilly, MD, Vera P. Shively, MS, William R. Flinn, MD, James S.T. Yao, MD, PhD  Journal of Vascular Surgery  Volume 10, Issue 4, Pages (October 1989) DOI: / (89) Copyright © 1989 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig. 1 a, Carboxymethyl-cellulose chromatography of a CB digestion of abdominal aortic aneurysm. The peak labeled CB8 was pooled for separation by agarose A-1.5 m chromatography. b, Agarose A-1.5 m molecular sieve chromatography of the CB peptides eluting in the CB8 region from carboxymethyl-cellulose. The collagen peptides α1(I)-CB(8-3), α1(I)-CB8, and α1(III)-CB8 eluted from the column at positions corresponding to molecular weight of 37,000, 24,000, and 12,000, respectively. Peak areas were used to calculate the ratios of type I and type III collagens. Journal of Vascular Surgery  , DOI: ( / (89) ) Copyright © 1989 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig. 2 Collagen and elastin concentration of infrarenal aorta from nonaneurysmal age-matched control subjects and abdominal aortic aneurysms, expressed as percentage delipidized, decalcified dry weight. Collagen weight was derived from hydroxyproline analysis. Journal of Vascular Surgery  , DOI: ( / (89) ) Copyright © 1989 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig. 3 A, Hematoxylin-eosin stain of infrarenal aorta from a nonaneurysmal age-matched control subject.B, A routine abdominal aortic aneurysm oriented with the intima superiorly and the adventitia inferiorly. Note the infiltration of inflammatory cells particularly within the adventitia of the aneurysm. (Original magnification × 16.) Journal of Vascular Surgery  , DOI: ( / (89) ) Copyright © 1989 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5 Fig. 4 A, Elastin van Gieson stain of infrarenal aorta from nonaneurysmal age-matched control subject.B, Abdominal aortic aneurysm oriented with the intima superiorly and the adventitia inferiorly. Note the deficiency and fragmentation of elastin (black) in the aneurysm. (Original magnification × 16.) Journal of Vascular Surgery  , DOI: ( / (89) ) Copyright © 1989 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

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