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Viability of primordial follicles derived from cryopreserved ovine ovarian cortex tissue
Patricia Miyuki Tsuribe, Ph.D., Carlos Alberto Monte Gobbo, M.D., Ph.D., Fernanda da Cruz Landim-Alvarenga, D.V.M., Ph.D. Fertility and Sterility Volume 91, Issue 5, Pages (May 2009) DOI: /j.fertnstert Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions
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Figure 1 Number of fragments in each treatment.
Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions
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Figure 2 A: Histological section of sheep fresh ovarian tissue showing normal preantral follicles (pf = primordial follicle, prf = primary follicle, o = oocyte) (original magnification ×400). B: Histological section of ovarian tissue showing degenerated primordial follicle with loss of granulosa cells (gc) and retraction of oocyte (arrow) (original magnification ×400). Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions
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Figure 3 Aspect of primordial follicles in culture. A: Aspect of a primary follicle after 6 days in culture. Observe the growth of cells of ovarian stroma (os). B: Aspect of two follicles considered degenerated in culture. Observe the absence of growth of cells of ovarian stroma and the darkened stain of oocytes (o) (original magnification ×200). Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions
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Figure 4 Aspect of a primary follicle after 10 days in culture. Observe the growth of ovarian stroma cells (os) and the presence of a basement membrane (mb) around the oocyte (o) (original magnification ×400). Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions
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