Presentation is loading. Please wait.

Presentation is loading. Please wait.

An Allele-Specific RT-PCR Assay to Detect Type A Mutation of the Nucleophosmin-1 Gene in Acute Myeloid Leukemia  Tiziana Ottone, Emanuele Ammatuna, Serena.

Published byErlin Darmali Modified over 5 years ago

Similar presentations

Presentation on theme: "An Allele-Specific RT-PCR Assay to Detect Type A Mutation of the Nucleophosmin-1 Gene in Acute Myeloid Leukemia  Tiziana Ottone, Emanuele Ammatuna, Serena."— Presentation transcript:

1 An Allele-Specific RT-PCR Assay to Detect Type A Mutation of the Nucleophosmin-1 Gene in Acute Myeloid Leukemia  Tiziana Ottone, Emanuele Ammatuna, Serena Lavorgna, Nélida I. Noguera, Francesco Buccisano, Adriano Venditti, Laura Giannì, Massimiliano Postorino, Giorgio Federici, Sergio Amadori, Francesco Lo-Coco  The Journal of Molecular Diagnostics  Volume 10, Issue 3, Pages (May 2008) DOI: /jmoldx Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 A: ASO-RT-PCR. An amplification band of 320 bp containing the NPM1-mutA is visualized in three patient samples (lanes 1, 2, and 3), whereas no amplification signal is visible in three patient samples with germline NPM1 (lanes 4, 5, and 6). B: successful amplification of the ABL gene used in all cases as internal PCR control. M, molecular weight marker. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Electropherograms of an NPM1-mut (A) and an NPM1-w/t (B) sample. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

4 Figure 3 Sequence traces showing the NPM1-mutA (A) and NPM1-w/t (B) pattern. The mutant sequence pattern in A contains the TCTG duplication. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

5 Figure 4 ASO-RT-PCR and semi-nested ASO-PCR of serial dilutions of NPM1-mutA. Lanes 1, 2, 3, and 4 correspond to the amplification by ASO-RT-PCR of 10−1, 10−2, 10−3, and 10−4 dilutions, respectively. Lanes 5, 6, 7, 8, 9, and 10 correspond to the amplification by semi-nested ASO-PCR of 10−1, 10−2, 10−3, 10−4, 10−5, and 10−6 dilutions. Lanes 11 and 12 correspond to the undiluted positive control and negative control, respectively. M, molecular weight marker. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

6 Figure 5 Results of semi-nested ASO-PCR analysis to amplify NPM1-mutA for MRD detection in four patients (a, b, c, and d) tested at diagnosis and during follow-up. The figure represents NPM1-mutA (▪) and NPM1-w/t (□) results of the semi-nested ASO-PCR assay. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Download ppt "An Allele-Specific RT-PCR Assay to Detect Type A Mutation of the Nucleophosmin-1 Gene in Acute Myeloid Leukemia  Tiziana Ottone, Emanuele Ammatuna, Serena."

Similar presentations

Cytogenetic and molecular diagnostic characterization combined to postconsolidation minimal residual disease assessment by flow cytometry improves risk.

Cytogenetic and molecular diagnostic characterization combined to postconsolidation minimal residual disease assessment by flow cytometry improves risk.
Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.
Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,

Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,
An Allele-Specific RT-PCR Assay to Detect Type A Mutation of the Nucleophosmin-1 Gene in Acute Myeloid Leukemia Tiziana Ottone, Emanuele Ammatuna, Serena.

An Allele-Specific RT-PCR Assay to Detect Type A Mutation of the Nucleophosmin-1 Gene in Acute Myeloid Leukemia Tiziana Ottone, Emanuele Ammatuna, Serena.
Margaret L. Gulley, Thomas C. Shea, Yuri Fedoriw 

Margaret L. Gulley, Thomas C. Shea, Yuri Fedoriw 
Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis  Philippe Szankasi, David.

Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis  Philippe Szankasi, David.
Alexander A. Morley, Sue Latham, Michael J. Brisco, Pamela J

Alexander A. Morley, Sue Latham, Michael J. Brisco, Pamela J
An Enhanced Polymerase Chain Reaction Assay to Detect Pre- and Full Mutation Alleles of the Fragile X Mental Retardation 1 Gene  Alessandro Saluto, Alessandro.

An Enhanced Polymerase Chain Reaction Assay to Detect Pre- and Full Mutation Alleles of the Fragile X Mental Retardation 1 Gene  Alessandro Saluto, Alessandro.
EGFR Mutations in Lung Adenocarcinomas

EGFR Mutations in Lung Adenocarcinomas
Detection of Exon 12 Mutations in the JAK2 Gene

Detection of Exon 12 Mutations in the JAK2 Gene
Denaturing High-Performance Liquid Chromatography

Denaturing High-Performance Liquid Chromatography
Todd S. Laughlin, Michael W. Becker, Jane L. Liesveld, Deborah A

Todd S. Laughlin, Michael W. Becker, Jane L. Liesveld, Deborah A
Margaret L. Gulley, Thomas C. Shea, Yuri Fedoriw 

Margaret L. Gulley, Thomas C. Shea, Yuri Fedoriw 
Analysis of T-Cell Clonality Using Laser Capture Microdissection and High-Resolution Microcapillary Electrophoresis  Evgeny Yakirevich, Cynthia L. Jackson,

Analysis of T-Cell Clonality Using Laser Capture Microdissection and High-Resolution Microcapillary Electrophoresis  Evgeny Yakirevich, Cynthia L. Jackson,
Comprehensive Analysis of CBFβ-MYH11 Fusion Transcripts in Acute Myeloid Leukemia by RT-PCR Analysis  ShriHari S. Kadkol, Annette Bruno, Carol Dodge,

Comprehensive Analysis of CBFβ-MYH11 Fusion Transcripts in Acute Myeloid Leukemia by RT-PCR Analysis  ShriHari S. Kadkol, Annette Bruno, Carol Dodge,
Jonathan A. Schumacher, Stephen D. Jenson, Kojo S. J

Jonathan A. Schumacher, Stephen D. Jenson, Kojo S. J
Isothermal Multiple Displacement Amplification

Isothermal Multiple Displacement Amplification
Molecular Monitoring of Residual Disease in Chronic Myeloid Leukemia by Genomic DNA Compared with Conventional mRNA Analysis  Elia Mattarucchi, Orietta.

Molecular Monitoring of Residual Disease in Chronic Myeloid Leukemia by Genomic DNA Compared with Conventional mRNA Analysis  Elia Mattarucchi, Orietta.
Establishment and Study of Different Real-Time Polymerase Chain Reaction Assays for the Quantification of Cells with Deletions of Chromosome 7  Elia Mattarucchi,

Establishment and Study of Different Real-Time Polymerase Chain Reaction Assays for the Quantification of Cells with Deletions of Chromosome 7  Elia Mattarucchi,
A DNA Real-Time Quantitative PCR Method Suitable for Routine Monitoring of Low Levels of Minimal Residual Disease in Chronic Myeloid Leukemia  Paul A.

A DNA Real-Time Quantitative PCR Method Suitable for Routine Monitoring of Low Levels of Minimal Residual Disease in Chronic Myeloid Leukemia  Paul A.

Similar presentations

Ads by Google