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Transfection of pulmonary artery segments in lung isografts during storage  Motoki Yano, MD, Masafumi Hiratsuka, MD, Bassem N Mora, MD, Ronald K Scheule,

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Presentation on theme: "Transfection of pulmonary artery segments in lung isografts during storage  Motoki Yano, MD, Masafumi Hiratsuka, MD, Bassem N Mora, MD, Ronald K Scheule,"— Presentation transcript:

1 Transfection of pulmonary artery segments in lung isografts during storage 
Motoki Yano, MD, Masafumi Hiratsuka, MD, Bassem N Mora, MD, Ronald K Scheule, PhD, G.Alexander Patterson, MD  The Annals of Thoracic Surgery  Volume 68, Issue 5, Pages (November 1999) DOI: /S (99)

2 Fig 1 Relative density of chloramphenicol acetyl transferase activity. Mean density of monoacetylated and diacetylated forms of chloramphenicol from thin-layer chromatography was measured using the NIH Image program. The density found in proximal pulmonary artery segments (PPASs) stored for 24 hours in group 4 was established as the standard (100%) against which to compare the other groups. The PPASs stored at 37°C in groups 2 and 3 showed gross transgene expression, and the expression increased successively. In group 4, similar and gross expression appeared in all PPASs. The Annals of Thoracic Surgery  , DOI: ( /S (99) )

3 Fig 2 Quantification of chloramphenicol acetyl transferase (CAT) by enzyme-linked immunosorbent assay. The quantity of CAT enzyme was measured in nanograms per segment of pulmonary artery (ng/PA). The proximal pulmonary artery segments stored for 24 hours at 37°C in group 2 showed a high level of CAT. In group 3, CAT was detected and increased after 12 hours’ storage. In group 4, CAT was detected with a high standard deviation. The Annals of Thoracic Surgery  , DOI: ( /S (99) )


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