Download presentation
Presentation is loading. Please wait.
Published byCameron Gibbs Modified over 5 years ago
1
Transfection of pulmonary artery segments in lung isografts during storage
Motoki Yano, MD, Masafumi Hiratsuka, MD, Bassem N Mora, MD, Ronald K Scheule, PhD, G.Alexander Patterson, MD The Annals of Thoracic Surgery Volume 68, Issue 5, Pages (November 1999) DOI: /S (99)
2
Fig 1 Relative density of chloramphenicol acetyl transferase activity. Mean density of monoacetylated and diacetylated forms of chloramphenicol from thin-layer chromatography was measured using the NIH Image program. The density found in proximal pulmonary artery segments (PPASs) stored for 24 hours in group 4 was established as the standard (100%) against which to compare the other groups. The PPASs stored at 37°C in groups 2 and 3 showed gross transgene expression, and the expression increased successively. In group 4, similar and gross expression appeared in all PPASs. The Annals of Thoracic Surgery , DOI: ( /S (99) )
3
Fig 2 Quantification of chloramphenicol acetyl transferase (CAT) by enzyme-linked immunosorbent assay. The quantity of CAT enzyme was measured in nanograms per segment of pulmonary artery (ng/PA). The proximal pulmonary artery segments stored for 24 hours at 37°C in group 2 showed a high level of CAT. In group 3, CAT was detected and increased after 12 hours’ storage. In group 4, CAT was detected with a high standard deviation. The Annals of Thoracic Surgery , DOI: ( /S (99) )
Similar presentations
© 2025 SlidePlayer.com. Inc.
All rights reserved.