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Biochemical characterization of the protein phosphatases Saci-PTP.

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Presentation on theme: "Biochemical characterization of the protein phosphatases Saci-PTP."— Presentation transcript:

1 Biochemical characterization of the protein phosphatases Saci-PTP.
Biochemical characterization of the protein phosphatases Saci-PTP. The activity of Saci-PTP was analyzed in the presence of pNPP (A), of the phospho-Tyr peptide TEVGKRI(pY)RLV (B), the phospho-Ser peptide NIDAIRA(pS)LNIMSR (C), and the phospho-Thr peptide ETTYERW(pT)TITQRER (D). All experiments were performed at 70 °C in triplicate. Saci_PTP showed general phosphatase activity with pNPP as substrate (A). Furthermore, Saci-PTP displayed a dual-specific phosphatase activity using p-Tyr peptides (B) as well as p-Ser (C) and p-Thr (D) peptides as substrate. Julia Reimann et al. Mol Cell Proteomics 2013;12: © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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