1. Reduction of Cd in Rice through Expression of OXS3-like Gene Fragments
Changhu Wang, Weili Guo, Shan Ye, Pengcheng Wei, David W. Ow 
Molecular Plant 
Volume 9, Issue 2, Pages (February 2016)
DOI: /j.molp
Copyright © 2016 The Author Terms and Conditions

2. Figure 1
Cd Tolerance and/or Accumulation in S. pombe or Rice by OsO3L2, OsO3L23, or Their Truncated Derivatives.
(A) Tolerance to Cd (0.35 mM) or diamide (2.0 mM) in S. pombe. -, growth not observed; +, ++, +++, ++++, growth observed at 1, 10−1, 10−2, 10−3 dilutions, respectively. Number shows amino acid residue position; shaded box indicates putative N-acetyltransferase catalytic domain.
(B) Representative phenotypes of 21-day-old rice seedlings grown 14 days in 20 μM CdCl2-containing nutrition solution (see also Supplemental Figure 3). Transgenic plants derived from Oryza sativa var. japonica cv. Zhonghua 11. Transgenic rice (Zhonghua 11) line ID shown in parentheses. WT, wild-type.
(C–E) T2 plants grown until ripening stage in 1.5 mg Cd/kg of DW soil (see Supplemental Methods): (C) biomass, (D) Cd concentration in shoot and root, and (E) Cd concentration in T3 dehusked grain from T2 plants; dashed gray lines indicate maximum recommended level for USA/Japan or China. (F) Cd concentration in T3 seedlings grown 7 days in solution then transferred to fresh solution containing 0, 20, or 30 μM CdCl2 for another 14 days. Transgenic rice (Zhonghua 11) line ID shown within (C and D), below (E), or above (F) each bar. Values are mean ± SD; n = 5. *p < 0.05, Dunnett's t-test. WT, wild-type.
(G) Yeast two-hybrid assay shows that O3L2/O3L3 interacts with histone H2A.
(H) 2B::DsRed and 3D::DsRed co-localize with histone H2A::GFP in subnuclear regions in onion epidermal cells. Scale bars, 20 μm.
Molecular Plant 2016 9, DOI: ( /j.molp )
Copyright © 2016 The Author Terms and Conditions