1. Antisense transcription, measurement, and correction
Antisense transcription, measurement, and correction Transcription profiles for both sense (gray) and antisense (red) strands for circuit grown in culture tube conditions for the −/+/− combination of inducers (22 nM aTc). Terminators are shown by light gray‐labeled regions. The antisense profiles for all combinations of inducers are shown in Appendix Fig S5. The profiles correspond to a single experiment that is representative of three replicates.The change in the transcription profile that occurs due the addition of a reverse terminator is shown. Shaded regions denote terminator part boundaries. The original terminator is L3S2P24, and this is replaced by the terminators ECK (forward) and ECK (reverse). The profile normalization (Box 1) has been applied to the 3′‐end of the antisense profile in the modified part.
Thomas E Gorochowski et al. Mol Syst Biol 2017;13:952
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