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Endemic meningococcal disease in Cerdanyola, Spain, 1987–93: molecular epidemiology of the isolates of Neisseria meningitidis  M. Esther Verdú, Pere Coll,

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Presentation on theme: "Endemic meningococcal disease in Cerdanyola, Spain, 1987–93: molecular epidemiology of the isolates of Neisseria meningitidis  M. Esther Verdú, Pere Coll,"— Presentation transcript:

1 Endemic meningococcal disease in Cerdanyola, Spain, 1987–93: molecular epidemiology of the isolates of Neisseria meningitidis  M. Esther Verdú, Pere Coll, Dionisia Fontanals, Francesca March, Immaculada Pons, Isabel Sanfeliu, Guillem Prats  Clinical Microbiology and Infection  Volume 2, Issue 3, Pages (December 1996) DOI: /S X(14) Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions

2 Figure 1 Genetic relationships of electrophoretic types (ETs) of N. meningitidis strains. The dendrogram was generated by the average-linkage method of clustering from matrix of coefficients of pairwise genetic distance (based on seven enzyme loci) for 13 ETs. Strain number, antigenic phenotypes (APs), pulse types (PTs) and electrophoretic types (ETs) are indicated. Clinical Microbiology and Infection 1996 2, DOI: ( /S X(14) ) Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions

3 Figure 2 PFGE separation of N. meningitidis genomic DNA. DNAs cleaved with the endonuclease NheI were separated into 16 different pulse types (PTs) by PFGE. (A) Lane 1, strain (PT A1); lane 2, strain (PT E); lane 3, strain (PT A4); lane 4, strain (PT F); lane 5, strain (PT A8); lane 6, strain (PT A7); lane 7, strain (PT C); lane 8, strain (PT I1); lane 9, strain (PT I2); lane 10, strain (PT II). (B) Lane 1, strain (PT A5); lane 2, strain (PT A2); lane 3, strain (PT A3); lane 4, strain (PT A6); lane 5, strain (PT B). Lanes M, standards (lambda concatemer). Sizes (kb) of standard DNA molecules are indicated. The strain (PT D) is not shown. Clinical Microbiology and Infection 1996 2, DOI: ( /S X(14) ) Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions

4 Figure 3 Dendrogram showing similarities between the NheI pulse types of the 30 N. meningitidis strains on the basis of the results of PFGE analysis. The dendrogram was generated by the UPGMA method of clustering from matrix of Dice similarity coefficients. The similarity among the patterns is indicated below the dendrogram as a percentage. Strain number, antigenic phenotypes (APs), pulse types (PTs) and electrophoretic types (ETs) are also indicated. Clinical Microbiology and Infection 1996 2, DOI: ( /S X(14) ) Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions

5 Figure 4 Similarity matrix of the closely or possibly related pulse types (PTs) of serogroup B isolates from Cerdanyola during 1987–93. The value of the Dice similarity coefficient (DSC) between 63% and 95% is depicted by three different tones in the matrix. Clinical Microbiology and Infection 1996 2, DOI: ( /S X(14) ) Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions


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