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G. Plumton, I. Adjei, A. Martin-Pena, G.D. Palmer, B. Sharma 

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Presentation on theme: "G. Plumton, I. Adjei, A. Martin-Pena, G.D. Palmer, B. Sharma "— Presentation transcript:

1 Macroporous 3D scaffolds for studying MSC migration and differentiation within cartilage defects 
G. Plumton, I. Adjei, A. Martin-Pena, G.D. Palmer, B. Sharma  Osteoarthritis and Cartilage  Volume 24, Pages S162-S163 (April 2016) DOI: /j.joca Copyright © Terms and Conditions

2 Figure 1 SEM Image of nanoporous and macroporoushydrogels. 0%, 10%, 20%, and 30% weight per volume gelatin microspheres (diamter 150–300 μm were suspended in solution to demonstrate variable porosity and architecture. Osteoarthritis and Cartilage  , S162-S163DOI: ( /j.joca ) Copyright © Terms and Conditions

3 Figure 2. Luminosity at three time points of cell media containing secreted luciferase produced by transfected cells. No luminosity registered in the control groups without virus. Osteoarthritis and Cartilage  , S162-S163DOI: ( /j.joca ) Copyright © Terms and Conditions

4 Figure 3 Left: GFP fluorescing cells (green)in a macroporoushydrogel. Right: MSCs in a PEGDA hydrogel transduced with a lentiviral reporter construct, constitutively expressingtdTomato (red), and with GFP production linked to the Collagen 2 promoter. Osteoarthritis and Cartilage  , S162-S163DOI: ( /j.joca ) Copyright © Terms and Conditions


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