1. Quantitative RT-PCR results of relative gene expression normalized to wild-type (WT) expression levels for the LjMYB14 transgene (A) and genes specific for isoflavonoid biosynthesis up-regulated in transgenic lines (B).
Quantitative RT-PCR results of relative gene expression normalized to wild-type (WT) expression levels for the LjMYB14 transgene (A) and genes specific for isoflavonoid biosynthesis up-regulated in transgenic lines (B). Expression levels of all genes were calculated relative to actin, and the increase in gene expression in the transgenic plants is expressed as fold change normalized to relative expression in wild-type MG-20. * Significant at P < 0.05 and ** significant at P < 0.01 compared with wild-type levels.
Dale Shelton et al. Plant Physiol. 2012;159:
©2012 by American Society of Plant Biologists