1. Volume 19, Issue 4, Pages 261-268 (September 2012)
Anemia associated with extraerythrocytic oxidative stress damage mediated by neutrophil superoxide anion production in chronic renal failure patients undergoing hemodialysis 
Jair Tonon, Flávia Alessandra Guarnier, Alessandra Lourenço Cecchini, Rubens Cecchini 
Pathophysiology 
Volume 19, Issue 4, Pages (September 2012)
DOI: /j.pathophys
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2. Fig. 1
Erythrocyte lipid peroxidizability (A). Erythrocytes were incubated with tert-butyl hydroperoxide, and lipid peroxidation was measured using TBARS test. A Boltzmann sigmoidal equation was applied to find the maximal lipid peroxidizability levels. Linear regression was applied to the linear part of the ascending curve to determine the initial velocity of lipid peroxidizability. Neutrophil superoxide generation (B). The reaction was started by the addition of phorbol myristate acetate, while superoxide was measured by cytochrome c reduction. The hyperbolic asymptotic equation was used to determine the maximal superoxide production and initial velocity.
Pathophysiology  , DOI: ( /j.pathophys )
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3. Fig. 2
Individual values of plasma TBARS levels and superoxide initial velocity formation from patients subjected to hemodialysis sections. TBARS value was expressed in μM and superoxide value was expressed in nmol/106cells/min. MDA, malondialdehyde.
Pathophysiology  , DOI: ( /j.pathophys )
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4. Fig. 3
A positive Pearson correlation between (A) “initial velocity” of neutrophil superoxide formation and erythrocyte lipid peroxidizability induced by tert-butyl hydroperoxide obtained at 40-min incubation time and (B) between hemoglobin levels and erythrocyte lipid peroxidizability. Points represent results from patients before dialysis.
Pathophysiology  , DOI: ( /j.pathophys )
Copyright © 2012 Elsevier Ireland Ltd Terms and Conditions