1. Subchondral bone changes in three different canine models of osteoarthritis 
K. Kuroki, C.R. Cook, J.L. Cook 
Osteoarthritis and Cartilage 
Volume 19, Issue 9, Pages (September 2011)
DOI: /j.joca
Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

2. Fig. 1
The medial and lateral tibias and the medial and lateral femoral condyles were each divided into four 3 mm-thick osteochondral slabs and the central two slabs (∗) obtained from each of four major weight-bearing compartments were histologically and histomorphometrically examined. M: medial. L: lateral.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

3. Fig. 2
Image analyses: a representative H&E stained osteochondral tissue section (left) and its bone thresholding image (right). The subchondral plate (A: lighter gray) and trabecular bone in the template placed in the epiphysis subjacent to the subchondral plate (B: gray rectangle area) were subjected to histomorphometric analyses.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

4. Fig. 3
Mean (95% confidence interval) lameness scores. ACLt dogs (n = 5) had significantly higher lameness scores than SH control dogs (n = 5) throughout the study period (∗P < 0.001 to P = 0.003). Lameness scores of GR dogs (n = 6) and MR dogs (n = 5) were not different from SH.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

5. Fig. 4
Representative photomicrographs of articular cartilage of the medial tibia (MT), lateral tibia (LT), medial femoral condyle (MF) and lateral femoral condyle (LF) and synovium. ACLt knees had one or combination of the following changes in all four areas examined: surface irregularity, fibrillation, clefts, chondrocyte cloning, and reduction of toluidine blue staining. GR knees had cartilage loss in the medial femoral condyle and reduction of toluidine blue staining with surface fibrillation in the medial tibia while MR knees had marked cartilage pathology in the medial compartment. In SH knees, the surface of the superficial cartilaginous layer was smooth, and the cartilage matrix was well stained with toluidine blue. In synovium there was marked hyperplasia with frequent lymphoid proliferation in ACLt knees. In GR knees, the synovium was lined by a single layer of plump synoviocytes with rare papillary projections. There was moderate synovial hyperplasia with infiltrates of small numbers of lymphocytes in MR knees. In SH knees, the synovium was lined by a single layer of flat synoviocytes with no overt inflammatory changes. H&E stain.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

6. Fig. 5
Representative thresholding images of subchondral bone of the medial tibia (MT), lateral tibia (LT), medial femoral condyle (MF) and lateral femoral condyle (LF). Subchondral plate and trabecular bone in ACLt are apparently thinner than any other groups in all four compartments examined.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

7. Fig. 6
Mean (95% confidence interval) synovitis scores. ACLt dogs (n = 5) had significantly more severe synovitis than SH control dogs (n = 5) (∗P < 0.001). Synovitis scores of GR dogs (n = 6) and MR dogs (n = 5) were not different from SH.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

8. Fig. 7
Correlations between lameness scores and Sp.Th (A), BV/TV (B), and trabecular thickness (Tb.Th) (C) within each weight-bearing compartment where data from all dogs (n = 21) are included. Lameness scores used in these figures are the scores at 8 weeks after surgery. Correlations between Mankin scores and Sp.Th within each OA model (ACLt: n = 5, GR: n = 6, and MR: n = 5) where data from all four compartments are included. A trendline is shown if a correlation is statistically significant (P < 0.05).
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions