1. This month in Gastroenterology
Karen Kim, Marc Bissonnette 
Gastroenterology 
Volume 126, Issue 1, Pages 1-2 (January 2004)
DOI: /j.gastro

2. Figure 1
The cumulative probability of an infectious complication relative to the number of infliximab infusions in 500 patients.
Gastroenterology  , 1-2DOI: ( /j.gastro )

3. Figure 2
Akt1, Akt2, and p-Akt are differentially distributed in native rabbit ileum. Localization of (A) p-Akt, (B) Akt1, and (C and D) Akt2 by immunohistochemistry in ileum. (D) Enlargement of a portion of panel C.
Gastroenterology  , 1-2DOI: ( /j.gastro )

4. Figure 3
Effects of cAMP and H89 on TLCS- and GCDC-induced activation of the CD95 system. Hepatocytes were cultured for 24 hours and then exposed to control medium, TLCS (100 μmol/L), GCDC (100 μmol/L), or DB-cAMP (100 μmol/L). If indicated, the PKA-specific inhibitor H89 (5 μmol/L) or the PI-3 kinase-specific inhibitors LY (10 μmol/L) or wortmannin (100 nmol/L) were preincubated for 30 minutes. Samples were collected and either underwent Western blotting for EGFR-phosphorylation (1) or immunoprecipitation for CD95 with subsequent Western blotting for EGFR (2), phospho-serine/threonine/tyrosine (3), or DISC formation as indicated by caspase 8- and FADD-immunoblotting (4). Total CD95 served as loading control. (1) Bile salt-induced EGFR phosphorylation was detected after 10 minutes of bile salt addition and was inhibited by DB-cAMP in a H89-sensitive, but LY and wortmannin-insensitive, way. (2) EGFR/CD95 association induced by TLCS or GCDC was measured after 60 minutes of bile salt addition and was not affected by DB-cAMP. (3) CD95 phosphorylations were detected after a 60-minute incubation of bile salts and/or DBcAMP. TLCS and GCDC induced CD95-Tyr phosphorylation, whereas DB-cAMP induced phosphorylation of CD95 on Ser/Thr residues. When DB-cAMP was incubated together with TLCS or GCDC, bile salt-induced CD95-Tyr phosphorylation was inhibited, while CD95-Ser/Thr phosphorylation still occurred in a PKA inhibition-sensitive way. (4) DISC formation was measured 3 hours after bile salt addition. TLCS and GCDC induced caspase 8 and FADD association with CD95, which was abolished in an H89-sensitive way when TLCS or GCDC were incubated together with DB-cAMP. Inhibition of PI-3 kinase with either wortmannin or LY did not alter DB-cAMP-mediated effects on TLCS-induced activation of the CD95 system.
Gastroenterology  , 1-2DOI: ( /j.gastro )