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Published byViolet Greer Modified over 5 years ago
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Leishmania identification by PCR of Giemsa-stained lesion imprint slides stored for up to 36 years
Â.C. Volpini, M.J. Marques, S. Lopes dos Santos, G.L. Machado-Coelho, W. Mayrink, A.J. Romanha Clinical Microbiology and Infection Volume 12, Issue 8, Pages (August 2006) DOI: /j x Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions
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Fig. 1 Representative polyacrylamide 10% w/v gel of PCR products obtained with primers specific for the conserved region of Leishmania mkDNA. Lanes: 1, reference strain of Leishmania (Leishmania) amazonensis IFLA/BR/1967/PH8; 2, reference strain of Leishmania (Viannia) braziliensis MHOM/BR/1975/M2903; 3 and 4, DNA from lesions of two patients with American cutaneous leishmaniasis; 5–12, restriction fragment length polymorphism fragments obtained after endonuclease digestion of the amplicons from lanes 1–4 with HaeIII (lanes 5–8) or ApaLI (lanes 9–12). MM, 25-bp molecular size ladder; NC, DNA-negative control. Clinical Microbiology and Infection , DOI: ( /j x) Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions
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