1. Human Dendritic Cells as Targets of Dengue Virus Infection
Mary Marovitch, Geraldine Grouard-Vogel, Michael Eller, Boonrat Tassaneetrithep, Deborah Birx, Curtis Hayes, Sarah Schlesinger- Frankel 
Journal of Investigative Dermatology Symposium Proceedings 
Volume 6, Issue 3, Pages (December 2001)
DOI: /j x x
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Primary dengue infection in a Thai infant demonstrates the distribution and hemorrhagic nature of the dengue rash.
Journal of Investigative Dermatology Symposium Proceedings 2001 6, DOI: ( /j x x)
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Immunofluorescence and flow cytometry of DV-infected DC. Immunofluorescence staining of (a) macrophages and (b) DC infected with DEN-2 (NGC) virus at an MOI of 0.2 with MoAb 3H5 and an FITC-conjugated secondary antibody (green). Evan's blue was used as a counterstain (red). (c) Flow cytometry of DEN-2 (NGC) exposed immature and mature DC populations stained with 3H5 (structural protein) and 7E11 (nonstructural protein indicative of replication) MoAb. (d) Flow cytometry of immature DC exposed to three different serotypes of primary dengue isolates. Dengue antigens were detected on day 2. Scale bar: 20 µm.
Journal of Investigative Dermatology Symposium Proceedings 2001 6, DOI: ( /j x x)
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Flow cytometry application of the ADE assay using immune serum. (a) ADE assay comparing K562 cells (positive control cells) and immature DC after exposure to DV that had been incubated with various dilutions of immune serum. Abscissa indicates intensity infection as determined by binding of 2H2-FITC (structural protein) and ordinate is the side scatter (SSC) in each dot plot. (b) Fc receptor expression on immature DC and K562 cells as determined by binding of specific MoAb of each receptor and analyzed using flow cytometry. Graphs show the mean percentage of cells staining positive for the respective Fc receptor of at least three independent experiments (mean ± SD).
Journal of Investigative Dermatology Symposium Proceedings 2001 6, DOI: ( /j x x)
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

5. Figure 4
TNF-α production by dengue-exposed DC induces DC maturation. (a) TNF-α protein detected (ELISA) in culture supernatants 24h after DV exposure. Virus concentration indicated on abscissa and TNF-α in pg per ml plotted on ordinate. (b) CD83 expression on DC exposed to DEN-2 (black line on histogram) in the presence of 20 µg per ml anti-TNF-α antibody or IgG control antibody. Green line is the mock infected (media alone) DC.
Journal of Investigative Dermatology Symposium Proceedings 2001 6, DOI: ( /j x x)
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

6. Figure 5
Infection of skin DC in DV vaccine recipient. (a–d) Skin biopsies from the lower extremity rash area. (a) Hematoxylin and eosin staining of formalin fixed, paraffin-embedded sections showing a mild, superficial, dermal perivascular lymphocytic infiltrate. (b) CD1a immunohistochemistry staining of a serial section of that in (a) showing enlarged cell bodies and processes of intraepithelial Langerhans' cells. (c) Frozen section stained with 2H2, a MoAb specific for envelope glycoprotein, shows two red cells containing DV glycoprotein. (d) Double labeling of frozen section using a Langerhans' cell marker, CD1a (blue) and a DV antigen, 2H2 (red). Scale bars: (a) 10 µm, (b) 15 µm, (c, d) 5 µm.
Journal of Investigative Dermatology Symposium Proceedings 2001 6, DOI: ( /j x x)
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions