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Nuclear volume differences between balanced and unbalanced spermatozoa in chromosomal translocation carriers Alexandre Rouen, Alinoë Lavillaureix, Capucine Hyon, Solveig Heide, Sylvain Clède, Richard Balet, Esther Kott, Nino Guy Cassuto, Jean-Pierre Siffroi Reproductive BioMedicine Online Volume 30, Issue 3, Pages (March 2015) DOI: /j.rbmo Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions
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Figure 1 Main steps of the study's protocol. After DAPI staining of the spermatozoa, their nuclear volumes are measured through confocal microscopy and computer analysis (ImageJ software). Secondly, a FISH assays allows for the assessment of the segregation mode of each spermatozoon. Reproductive BioMedicine Online , DOI: ( /j.rbmo ) Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions
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