Presentation is loading. Please wait.

Presentation is loading. Please wait.

Characterization of the inflammatory and apoptotic cells in the aortas of patients with ascending thoracic aortic aneurysms and dissections  Rumin He,

Published byBella Berg Modified over 5 years ago

Similar presentations

Presentation on theme: "Characterization of the inflammatory and apoptotic cells in the aortas of patients with ascending thoracic aortic aneurysms and dissections  Rumin He,"— Presentation transcript:

1 Characterization of the inflammatory and apoptotic cells in the aortas of patients with ascending thoracic aortic aneurysms and dissections  Rumin He, MD, Dong-Chuan Guo, PhD, Anthony L. Estrera, MD, Hazim J. Safi, MD, Tam T. Huynh, MD, Zhengnan Yin, MD, PhD, Shi- Nian Cao, MD, Jing Lin, MD, Thomas Kurian, L. Maximillian Buja, MD, Yong-Jian Geng, MD, PhD, Dianna M. Milewicz, MD, PhD  The Journal of Thoracic and Cardiovascular Surgery  Volume 131, Issue 3, Pages e2 (March 2006) DOI: /j.jtcvs Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

2 Figure 1 T-lymphocyte infiltrates in TAA and TAD. For orientation, lumen of aorta is at top with adventitia at bottom. A through C, Immunohistochemical staining demonstrates that CD3+ cells (arrow) are rarely present in control aorta. D, F, and G, Media from patients with TAA and TAD demonstrate CD3+ cells (arrows) throughout media of aorta. E, Adventitia from patient with TAA demonstrates CD3+ cells throughout adventitia. H through J, CD4+ and CD8+ cells are present in aortic media from patients with TAA and TAD. K and L, Media from patient with TAA demonstrates that CD1α+ and CD68+ cells are present in aneurysmal aortic media. Monoclonal anti-CD3 antibody used in D was obtained from DAKO Corporation, Carpinteria, Calif; antibodies in panels A through C and E through F were obtained from Zymed Laboratories Inc, South San Francisco, Calif, for comparison. Original magnification 400×. The Journal of Thoracic and Cardiovascular Surgery  , e2DOI: ( /j.jtcvs ) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

3 Figure 2 CD3+ cells present in media from control, aneurysmal, and dissected aortas. CD3+ cells in 1 mm2 of aortic media were counted from aortic tissue sections from control subjects, patients with TAAs, and patients with TADs. Total areas examined were 2.2 mm2. Box-whisker plots display CD3. Central horizontal bars indicate median, upper and lower ends of boxes indicate 75th and 25th percentiles, and error bars indicate maximum and minimum values of CD3 values. P values were determined by Mann-Whitney Wilcoxon rank-sum tests. The Journal of Thoracic and Cardiovascular Surgery  , e2DOI: ( /j.jtcvs ) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

4 Figure 3 Increased TUNEL-positive and FasL staining in media of aneurysmal and dissected aortas. Top: A, TUNEL-positive cells (arrows) are rarely present in control aorta. B and C, Media from patients with TAA and TAD demonstrated TUNEL-positive cells (arrows). D through F, Double staining with combination of TUNEL and immunohistochemical assay with α-actin, CD3, and CD68 antibodies demonstrated that SMCs, T lymphocytes, and macrophages were undergoing apoptosis (arrows) in aortic media from patient with TAA. TUNEL staining in panels A through C and E used DeadEnd Colorimetric TUNEL System; that in panels D and F used DeadEnd Fluorescence TUNEL System. Bottom: A and C, Adventitia and media in aneurysmal aorta indicate influx of CD3+ cells in walls of vasa vasorum and aortic media. B and D, Same vasa vasorum and media were stained with anti-FasL antibody, which indicated colocation of CD3+ and FasL-positive cells. Magnification 400×. The Journal of Thoracic and Cardiovascular Surgery  , e2DOI: ( /j.jtcvs ) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

5 Figure 4 TUNEL-positive cells present in media from control, aneurysmal, and dissected aortas. TUNEL-positive cells in 1 mm2 of the aortic media were counted from aortic tissue sections from control patients, patients with TAAs, and patients with TADs. Total areas examined were 2.2 mm2. Box-whisker plots display TUNEL. Central horizontal bars indicate median, upper and lower ends of boxes indicate 75th and 25th percentiles, and error bars indicate maximum and minimum values of TUNEL values. P values were determined by Mann-Whitney Wilcoxon rank-sum tests. The Journal of Thoracic and Cardiovascular Surgery  , e2DOI: ( /j.jtcvs ) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

6 Figure E1 Medial degeneration of aorta associated with loss of SMCs and elastic fibers. Representative cross-sections of human control and aneurysmal aortic tissue were stained with hematoxylin and eosin (A-C) or with monoclonal antibody directed against SMC α-actin (D-F). A and D, Media from control aorta demonstrates nuclei and elastic fibers throughout media. B and E, Media from patient with TAA reveals loss of cell nuclei in band in media, but elastic fibers remain. C and F, Media in different field from same patient as in B and E displays areas with loss of both cells and nuclei. Magnification 100×. The Journal of Thoracic and Cardiovascular Surgery  , e2DOI: ( /j.jtcvs ) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

7 Figure E2 Immunoblot analysis showed levels of Fas, FasL, and activated caspase-3 to be increased in aneurysmal and dissected aortas. Levels of Fas and FasL were assessed relative to actin. Increased levels of Fas antigen were detected in aneurysmal (0.82 ± 0.39) and dissected (0.68 ± 0.62) tissues relative to control tissues (0.07). Increased levels of FasL antigen were detected in aneurysmal (0.71 ± 0.23) and dissected (0.42 ± 0.31) tissues relative to control tissues (0.15). Total protein loaded into each lane was 50 μg. After separation by sodium dodecylsulfate–polyacrylamide gel electrophoresis, membranes were blocked and incubated with primary antibody against Fas, FasL, and activated caspase-3. Membranes were incubated with horseradish peroxidase–linked secondary antibodies, followed by visualization with SuperSignal West Femto kit (Pierce Biotechnology, Inc, Rockford, Ill). Bands correspond to following molecular weighs of proteins: Fas, 40 kd; FasL, 37 kd; activated caspase-3, 20.4 kd. Results are consistent with results obtained with immunohistochemical testing. The Journal of Thoracic and Cardiovascular Surgery  , e2DOI: ( /j.jtcvs ) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

Download ppt "Characterization of the inflammatory and apoptotic cells in the aortas of patients with ascending thoracic aortic aneurysms and dissections  Rumin He,"

Similar presentations

Aprotinin improves kidney function and decreases tubular cell apoptosis and proapoptotic signaling after renal ischemia-reperfusion  Ajay Kher, MD, Kirstan.

Aprotinin improves kidney function and decreases tubular cell apoptosis and proapoptotic signaling after renal ischemia-reperfusion  Ajay Kher, MD, Kirstan.
Nondissecting aneurysm of the thoracic aorta with arteritis in systemic lupus erythematosus  Hisato Takagi, MD, PhDa, Yoshio Mori, MD, PhDa, Hisashi Iwata,

Nondissecting aneurysm of the thoracic aorta with arteritis in systemic lupus erythematosus  Hisato Takagi, MD, PhDa, Yoshio Mori, MD, PhDa, Hisashi Iwata,
Expression of molecular mediators of apoptosis and their role in the pathogenesis of lower-extremity varicose veins  Enrico Ascher, MD, Theresa Jacob,

Expression of molecular mediators of apoptosis and their role in the pathogenesis of lower-extremity varicose veins  Enrico Ascher, MD, Theresa Jacob,
Giant thrombus in ascending aorta and aortic arch

Giant thrombus in ascending aorta and aortic arch
Azelnidipine suppresses the progression of aortic aneurysm in wild mice model through anti-inflammatory effects  Hirotsugu Kurobe, MD, PhD, Yuki Matsuoka,

Azelnidipine suppresses the progression of aortic aneurysm in wild mice model through anti-inflammatory effects  Hirotsugu Kurobe, MD, PhD, Yuki Matsuoka,
Mesenchymal stem cells attenuate angiotensin II-induced aortic aneurysm growth in apolipoprotein E-deficient mice  Ryotaro Hashizume, MD, Aika Yamawaki-Ogata,

Mesenchymal stem cells attenuate angiotensin II-induced aortic aneurysm growth in apolipoprotein E-deficient mice  Ryotaro Hashizume, MD, Aika Yamawaki-Ogata,
Mycoplasma hominis prosthetic valve endocarditis: The value of molecular sequencing in cardiac surgery  Syed T. Hussain, MD, Steven M. Gordon, MD, Carmela.

Mycoplasma hominis prosthetic valve endocarditis: The value of molecular sequencing in cardiac surgery  Syed T. Hussain, MD, Steven M. Gordon, MD, Carmela.
Accelerated aneurysmal dilation associated with apoptosis and inflammation in a newly developed calcium phosphate rodent abdominal aortic aneurysm model 

Accelerated aneurysmal dilation associated with apoptosis and inflammation in a newly developed calcium phosphate rodent abdominal aortic aneurysm model 
Tracheal regeneration: Myth or fact?

Tracheal regeneration: Myth or fact?
Bryce J. Laing, MD, David B. Ross, MD, Steven R

Bryce J. Laing, MD, David B. Ross, MD, Steven R
Coarctation-induced degenerative abdominal aortic aneurysm in a porcine model  Pao-Yen Lin, MD, Yeng-Ting Wu, MS, Guan-Cheng Lin, BS, Yao Hsiang Shih,

Coarctation-induced degenerative abdominal aortic aneurysm in a porcine model  Pao-Yen Lin, MD, Yeng-Ting Wu, MS, Guan-Cheng Lin, BS, Yao Hsiang Shih,
Prevention of the second stage of epithelial loss is a potential novel treatment for bronchiolitis obliterans  Yunge Zhao, MD, PhD, John F. Steidle, BA,

Prevention of the second stage of epithelial loss is a potential novel treatment for bronchiolitis obliterans  Yunge Zhao, MD, PhD, John F. Steidle, BA,
A proteomic study of the aortic media in human thoracic aortic dissection: Implication for oxidative stress  Mingfang Liao, MD, PhD, Zhaoyang Liu, PhD,

A proteomic study of the aortic media in human thoracic aortic dissection: Implication for oxidative stress  Mingfang Liao, MD, PhD, Zhaoyang Liu, PhD,
Experimental generation of a tissue-engineered functional and vascularized trachea  Thorsten Walles, MD, Bettina Giere, Michael Hofmann, PhD, Johanna Schanz,

Experimental generation of a tissue-engineered functional and vascularized trachea  Thorsten Walles, MD, Bettina Giere, Michael Hofmann, PhD, Johanna Schanz,
Inflammation-related induction of absent in melanoma 2 (AIM2) in vascular cells and atherosclerotic lesions suggests a role in vascular pathogenesis 

Inflammation-related induction of absent in melanoma 2 (AIM2) in vascular cells and atherosclerotic lesions suggests a role in vascular pathogenesis 
Exogenous surfactant attenuation of ischemia–reperfusion injury in the lung through alteration of inflammatory and apoptotic factors  Bart P. van Putte,

Exogenous surfactant attenuation of ischemia–reperfusion injury in the lung through alteration of inflammatory and apoptotic factors  Bart P. van Putte,
Deleted in Malignant Brain Tumors 1 is up-regulated in bacterial endocarditis and binds to components of vegetations  Hanna Müller, MD, Marcus Renner,

Deleted in Malignant Brain Tumors 1 is up-regulated in bacterial endocarditis and binds to components of vegetations  Hanna Müller, MD, Marcus Renner,
Histologic analysis of stent graft oversizing in the thoracic aorta

Histologic analysis of stent graft oversizing in the thoracic aorta
Increased vascularization of shoulder regions of carotid atherosclerotic plaques from patients with diabetes  Fredrik J. Olson, PhD, Sofia Strömberg,

Increased vascularization of shoulder regions of carotid atherosclerotic plaques from patients with diabetes  Fredrik J. Olson, PhD, Sofia Strömberg,
Transgenic model of cardiac rhabdomyosarcoma formation

Transgenic model of cardiac rhabdomyosarcoma formation

Similar presentations

Ads by Google