1. Bullous Pemphigoid, Neurodegenerative Disease, and Hippocampal BP180 Expression: A Retrospective Postmortem Neuropathologic Study 
Benjamin J. Barrick, Cristiane M. Ida, Valerie Laniosz, Mark E. Jentoft, Sindhuja Sominidi-Damodaran, Carilyn N. Wieland, Alexander Meves, Julia S. Lehman 
Journal of Investigative Dermatology 
Volume 136, Issue 10, Pages (October 2016)
DOI: /j.jid
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2. Figure 1
BP180 and AGRN expression in the hippocampus and skin. (a) Gene expression as determined by qRT-PCR. Oligonucleotides for BP180 and AGRN amplification were the following: (1) BP180-forward: 5′-TGTGAGGGTGTCAGAGAGCA-3′; (2) BP180-reverse: 5′-ACAGTGTAGGCCATCCCTTG-3′; (3) AGRN-forward: 5′-TGTGACCGAGAGCGAGAAG-3′; and (4) AGRN-reverse: 5′-CAGGCTCAGTTCAAAGTGGTT-3′. BP180 mRNA copy number is shown per 25,000 copies of housekeeping genes (ACTB, RPL8, and RPLP0). AGRN served as a positive control gene expressed in skin and hippocampus. (b) Examples of IHC of skin (positive control) and hippocampus using the indicated monoclonal antibodies targeting either the intracellular or extracellular NC16A domain of BP180. Antibody dilution 1:100 (EPR14758) or 1:25 (NC16A-3); heat induced epitope retrieval was with citrate-based buffer pH 5.9–6.1 (EPR14758) or EDTA-based buffer pH 8.9–9.1 (NC16A-3). Close-ups, right lower boxes. Scale bars: 500 μm. IHC, immunohistochemistry; qRT-PCR, quantitative reverse transcription polymerase chain reaction.
Journal of Investigative Dermatology  , DOI: ( /j.jid )
Copyright © 2016 The Authors Terms and Conditions