1. Transgenerational changes in somatic and germ line genetic integrity of first-generation offspring derived from the DNA damaged sperm 
Satish Kumar Adiga, Ph.D., Dinesh Upadhya, M.Sc., Guruprasad Kalthur, Ph.D., Satish Rao Bola Sadashiva, Ph.D., Pratap Kumar, M.D. 
Fertility and Sterility 
Volume 93, Issue 8, Pages (May 2010)
DOI: /j.fertnstert
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Radiation-induced DNA damage in mouse spermatozoa. Incidence of DNA damage in spermatozoa exposed to various doses of γ-radiation showing (A) denatured sperms as measured by the AO test. P<.01 for the 5-Gy versus the 0-Gy and 2.5-Gy groups, P<.001 for the 10-Gy versus the 0-Gy, 2.5-Gy, and 5-Gy groups. (B) Tail DNA as measured by the comet assay. P<.01 for 2.5 Gy versus 0 Gy, P<.001 for 0 Gy and 2.5 Gy versus 10 Gy.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Genetic instability in preimplantation embryos derived from the DNA-damaged sperm. The embryo derived from the sham-irradiated (0 Gy) sperm (▪), 2.5 Gy (●) 5 Gy (▴), and 10 Gy (▾) paternal irradiation showing micronuclei frequency at various preimplantation developmental periods. P<.001 for 5 Gy and 10 Gy versus 0 Gy on day 3.5.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Transgenerational genetic instability in somatic and germ cell compartment. (A)Micronuclei frequency in fetal liver cells derived from paternal sperm irradiation on day 18 of gestation, P<.001 for 5 and 10 Gy versus 0 Gy (B) Sperm denaturation level measured by AO assay showing percent denatured spermatozoa of F1 male offspring, P<.01 for the 0 and 10 Gy groups (C) Comet analysis of spermatozoa derived from the DNA-damaged sperm showing percent tail DNA, P<.001 for the 0-Gy and 10-Gy groups. The data were representative of three independent experiments.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

5. Figure 4
Association between preimplantation embryonic and somatic cell genetic instability in fetal liver cells. Regression analysis of micronuclei frequency in day 3.5 preimplantation embryos and fetal liver cells derived from the DNA damaged sperm. (A) 0 Gy, R = 0, P=NS, (B) 2.5 Gy, R = .09, P=NS, (C) 5 Gy, R = .50, P<.001, (D) 10 Gy, R = .74, P<.001.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions