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Supplemental Figure 2 RPMI8226 cells were incubated with increasing concentrations of the respective HIV PI (0-160 mM; Am: amprenavir; At: atazanavir;

Published byEsko Majanlahti Modified over 5 years ago

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Presentation on theme: "Supplemental Figure 2 RPMI8226 cells were incubated with increasing concentrations of the respective HIV PI (0-160 mM; Am: amprenavir; At: atazanavir;"— Presentation transcript:

1 Supplemental Figure 2 RPMI8226 cells were incubated with increasing concentrations of the respective HIV PI (0-160 mM; Am: amprenavir; At: atazanavir; D: darunavir; I: indinavir; T: tipranavir) and intracellular proteasome activity was assessed using the affinity probe MV151. The bargraphs represent relative changes in proteasome subunit-specific (b1/b5 vs. b2) fluorescence signals compared to untreated controls. RPMI MV-151 β- actin [µM] β1(i)/β5(i) β2 β2i [kD] 30 20 45 β2/2i β5/5i/1/1i Change in proteasome activity [%] NLVS NLVS Am At D I T V RPMI alle HIV-PI mono (MV –Gele vom 7.3., auswertung vom MV )

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